TY - JOUR TI - Localization of virus neutralizing I/LnJ H2-Oβ allele using confocal microscopy DO - https://doi.org/doi:10.7282/T3251NN6 PY - 2018 AB - Class II MHC peptide presentation is a key process in the adaptive immune response and maintenance of self-tolerance. The loading of peptides on to the class II MHC molecule is facilitated by the non-classical MHC molecule H2-M. The actions of H2-M are inhibited when complexed with another non-classical MHC molecule, H2-O. A functionally null H2-O molecule has been identified in the I/LnJ strain of mice; this strain has the ability to clear persistent retroviruses that other strains (B6 and BALB/cJ) cannot. This study aims to localize the I/LnJ H2-O peptide in dendritic cells using confocal microscopy in order to determine if mis-localization of free I/LnJ H2-O contributes to the ability of I/LnJ mice to produce retrovirus neutralizing antibodies. Preliminary data suggests that free I/LnJ H2-O is localized to compartments other than the endosome and endoplasmic reticulum, which has been previously reported. Data suggests that free I/LnJ H2-O may localize to mitochondria, which introduces the potential for a novel H2-O function in these mice. KW - Cell and Developmental Biology LA - eng ER -