Class II MHC peptide presentation is a key process in the adaptive immune response and maintenance of self-tolerance. The loading of peptides on to the class II MHC molecule is facilitated by the non-classical MHC molecule H2-M. The actions of H2-M are inhibited when complexed with another non-classical MHC molecule, H2-O. A functionally null H2-O molecule has been identified in the I/LnJ strain of mice; this strain has the ability to clear persistent retroviruses that other strains (B6 and BALB/cJ) cannot. This study aims to localize the I/LnJ H2-O peptide in dendritic cells using confocal microscopy in order to determine if mis-localization of free I/LnJ H2-O contributes to the ability of I/LnJ mice to produce retrovirus neutralizing antibodies. Preliminary data suggests that free I/LnJ H2-O is localized to compartments other than the endosome and endoplasmic reticulum, which has been previously reported. Data suggests that free I/LnJ H2-O may localize to mitochondria, which introduces the potential for a novel H2-O function in these mice.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_8849
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vi, 37 p. : ill.)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Jacqueline Katz
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.