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Understanding the anti-cancer activities of Moringa isothiocyanates in breast cancer cells
Descriptive
TitleInfo
Title
Understanding the anti-cancer activities of Moringa isothiocyanates in breast cancer cells
Name
(type = personal)
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(type = family)
Olayanju
NamePart
(type = given)
Julia Bolanle
NamePart
(type = date)
1976-
DisplayForm
Julia Bolanle Olayanju
Role
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(authority = RULIB)
author
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NamePart
(type = family)
Ganesan
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Shridar
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Shridar Ganesan
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Advisory Committee
Role
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chair
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(type = family)
Xia
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(type = given)
Bing
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Bing Xia
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Drake
NamePart
(type = given)
Justin
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Justin Drake
Affiliation
Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Walworth
NamePart
(type = given)
Nancy
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Nancy Walworth
Affiliation
Advisory Committee
Role
RoleTerm
(authority = RULIB)
outside member
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NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
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NamePart
School of Graduate Studies
Role
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school
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Text
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theses
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2018
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2018-05
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2018
Place
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xx
Language
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eng
Abstract
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Isothiocyanates (ITCs) are a class of naturally occurring compounds shown to have anti-cancer activities and promising chemopreventive prospects. Moringa oleifera (MO), a cruciferous vegetable widely cultivated in the tropics, has been used by locals to treat different diseases for centuries. Moringa Isothiocyanates (MIC) is produced from MO when the four unique sugar-modified aromatic glucosinolates it contains are hydrolyzed in a reaction catalyzed by myrosinase. In this study, we evaluated the efficacy of MIC on 9 different breast cancer cell lines and found it to be a potent anti-cancer agent. Interestingly, two of the most sensitive cells lines to MIC, BT474 and HCC1954, were both positive for HER2. HER2 is amplified in up to 30% of breast cancers and is positively correlated with poor prognosis in patients. We hypothesized that HER2 overexpression induces hypersensitivity to MIC in breast cancer cells. Our studies using MCF7 and MDA-MB-231 with and without HER2 overexpression further confirmed the correlation between HER2 overexpression and hypersensitivity to MIC. To understand why increased sensitivity to MIC was observed in HER2+ breast cancer cell lines as compared to HER2- cell lines, intracellular levels of reactive oxygen species (ROS) was examined. Higher basal intracellular ROS levels were observed in HER2+ cell lines compared to HER2- ones. We also hypothesized that the anti-cancer activity of MIC may involve an ROS regulatory pathway. Indeed, we found that MIC clearly regulates NRF2 and KEAP1 expression in multiple breast cancer cell lines. Additionally, MIC also triggers changes in intracellular ROS levels in a cell line-dependent manner, suggesting that MIC adopts different mechanisms of action in different breast cancer cell lines. Our work shows that MIC may open new frontiers in breast cancer prevention and therapy, particularly for those with HER2 amplification.
Subject
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(authority = ETD-LCSH)
Topic
Moringa oleifera
Subject
(authority = ETD-LCSH)
Topic
Breast--Cancer
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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ETD_8867
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xii, 87 p. : ill.)
Note
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Ph.D.
Note
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Includes bibliographical references
Note
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by Julia Bolanle Olayanju
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TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier
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doi:10.7282/T3W66Q7B
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
(type = personal)
Name
FamilyName
Olayanju
GivenName
Julia
MiddleName
Bolanle
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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2018-04-12 14:08:35
AssociatedEntity
Name
Julia Olayanju
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
DateTime
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2018-05-31
DateTime
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2018-11-30
Type
Embargo
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after November 30th, 2018.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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