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Role of TOP2B in photoreceptor gene regulatory network by single-cell transcriptome analysis
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Pinto, Alexandria. Role of TOP2B in photoreceptor gene regulatory network by single-cell transcriptome analysis. Retrieved from https://doi.org/doi:10.7282/T33R0X9T

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TitleRole of TOP2B in photoreceptor gene regulatory network by single-cell transcriptome analysis
NamePinto, Alexandria (author); Cai, Li (chair); Rutgers University; School of Graduate Studies
Date Created2018
Other Date2018-05 (degree)
SubjectBiomedical Engineering, DNA topoisomerase II
Extent1 online resource (ix, 58 p. : ill.)
DescriptionTOP2B is an enzyme that allows for access to the DNA strand for gene transcription. During development, TOP2B is found in cells which have finished mitosis and proliferation, suggesting its function in cell differentiation. Previously, bulk RNA-seq analysis of the retina revealed TOP2B controls expression of genes in the photoreceptor gene-regulatory network. However, bulk RNA-seq does not allow for direct analysis of individual cells to identify the role of TOP2B in photoreceptor cell differentiation. The central hypothesis is that grouping cells based on the photoreceptor gene regulatory network and applying bioinformatics analysis to the data can show that TOP2B plays an essential role in proper photoreceptor differentiation. In this study, we preform bioinformatics analysis on publically available single-cell RNA-seq (scRNA-seq) dataset of postnatal day 14 mouse retina (GSE63473) to determine to role of TOP2B in the photoreceptor gene regulatory network and identify novel genes which contribute to this pathway. Analysis of photoreceptor scRNA-seq data reveals that TOP2B expression is correlated with the expression of photoreceptor marker genes, confirming its role in photoreceptor differentiation. In addition, gene Fam19a3 was identified for its novel role contributing to the Top2b-controlled photoreceptor gene regulatory network. Thus, this study provides further insight into the photoreceptor differentiation processes that could be affected by the gene regulatory pathway.
NoteM.S.
NoteIncludes bibliographical references
Noteby Alexandria Pinto
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/T33R0X9T
Languageeng
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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