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The role of non-ionic surfactant-extracellular polymeric substances in removal of Listeria monocytogenes biofilm formed on different contact surfaces
Descriptive
TitleInfo
Title
The role of non-ionic surfactant-extracellular polymeric substances in removal of Listeria monocytogenes biofilm formed on different contact surfaces
Name
(type = personal)
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(type = family)
Albayati
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Firas S.
NamePart
(type = date)
1979-
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Firas S. Albayati
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(authority = RULIB)
author
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(type = personal)
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(type = family)
Takhistov
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(type = given)
Paul
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Paul Takhistov
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Advisory Committee
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chair
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Zylstra
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Gerben
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Gerben Zylstra
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Advisory Committee
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(authority = RULIB)
internal member
Name
(type = personal)
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(type = family)
Chikindas
NamePart
(type = given)
Michael
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Michael Chikindas
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Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
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(type = family)
Yam
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(type = given)
Kit
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Kit Yam
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Advisory Committee
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outside member
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Rutgers University
Role
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degree grantor
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School of Graduate Studies
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school
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Text
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theses
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2018-10
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2018
Place
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xx
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2018
Language
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eng
Abstract
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The occurrence of biofilm in food processing environments can lead to spoilage and transmission of diseases, this aided in the development of this study. Listeria monocytogenes can cause serious and possibly fatal illnesses in humans and animals after ingestion the contaminated food. Spinach can be contaminated by L. monocytogenes during harvest. The effectiveness of six nonionic surfactants (Pluronic F68, Pluronic F127, Tween 20, Tween 40, Tween 80, and Brij 58) were evaluated in disrupting L. monocytogenes biofilms on the surface of spinach leaves. Wells were washed with surfactants after incubation and then mixed on a platform shaker for 1, 5, 15, and 30 min. Then, the wells were rinsed with distilled water to remove dead cells, and fixation was conducted at 30 min at 60 °C. Our findings showed that Brij 58 most effectively removed the L. monocytogenes biofilm on spinach, followed by Pluronic F127, Tween 80, Tween 40, Tween 20, and Pluronic F68.In the second experiment, the effectiveness of different types of nonionic surfactants such as Pluronic F68, Pluronic F127, Tween 20, Tween 40, Tween 80 and Brij 58 against the Listeria monocytogenes biofilm cells formed on food contact surfaces made of Low-Density Polyethylene (LDPE), Polypropylene (PP), Low-Density Polyethylene and Polypropylene (LDPE-PP), Low-Density Polyethylene and Ethylene Vinyl Acetate (LDPE-EVA), Stainless-Steel and Aluminum was determined.Acridine Orange staining (AOS) to quantify the amount of the L. monocytogenes biofilm cells that were destroyed by the nonionic surfactants after different time intervals of 1, 5, 15 and 30 min.
Subject
(authority = RUETD)
Topic
Microbial Biology
Subject
(authority = LCSH)
Topic
Listeria monocytogenes
Subject
(authority = LCSH)
Topic
Extracellular polymeric substances
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
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(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Identifier
ETD_9118
Identifier
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doi:10.7282/T35X2DJV
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electronic resource
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application/pdf
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text/xml
Extent
1 online resource (xi, 112 pages : illustrations (some color))
Note
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Ph.D.
Note
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Includes bibliographical references
Note
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by Firas S. Albayati
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Genre
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ETD doctoral
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Albayati
GivenName
Firas S.
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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2018-07-26 02:14:47
AssociatedEntity
Name
firas Al-bayati
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Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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