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Comparison of amplicon sequencing to conventional fecal source tracking techniques in the Navesink River

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TitleInfo
Title
Comparison of amplicon sequencing to conventional fecal source tracking techniques in the Navesink River
Name (type = personal)
NamePart (type = family)
Phelan
NamePart (type = given)
Sarah
NamePart (type = date)
1988-
DisplayForm
Sarah Phelan
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Fahrenfeld
NamePart (type = given)
Nicole
DisplayForm
Nicole Fahrenfeld
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Guo
NamePart (type = given)
Quizong
DisplayForm
Quizong Guo
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Lifreri
NamePart (type = given)
James
DisplayForm
James Lifreri
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2018
DateOther (qualifier = exact); (type = degree)
2018-10
CopyrightDate (encoding = w3cdtf)
2018
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Fecal indicator bacteria are commonly used to evaluate water quality and make decisions on designating and restricting use. A drawback of this method is it does not differentiate fecal sources for management purposes. Microbial source tracking is being explored as an alternative and more informative method of assessing and identifying contaminant sources. High throughput sequencing of the 16s rRNA region allows for rapid, large scale microbial community analyses and is an attractive source tracking method that has not been thoroughly explored. To investigate the effectiveness of this method, samples were collected from representative fecal sources from horse, dogs, geese, and wastewater then mixed in known quantities to create a fecal library. A series of field samples were collected during wet and dry weather from the Navesink River, Red Bank, New Jersey. The library and field samples were evaluated for fecal coliform, qPCR for fecal marker genes, and those results were compared with the microbial community fingerprints generated by amplicon sequencing. The results from all three techniques were cross compared to understand the consistency of results between methods, and will inform the application of amplicon sequencing for source tracking in surface waters impacted by fecal/bacterial contamination. Results showed significant differences in library samples with biomarkers that could be applied with success to select sites with elevated coliform results; indicate further biomarker investigation is needed in sequencing results.
Subject (authority = RUETD)
Topic
Civil and Environmental Engineering
Subject (authority = ETD-LCSH)
Topic
Water quality biological assessment
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_9275
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (61 pages : illustrations)
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Sarah Phelan
Subject (authority = lcsh/lcnaf)
Geographic
Navesink River (N.J.)
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-dxm1-2n26
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Phelan
GivenName
Sarah
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2018-09-27 20:25:07
AssociatedEntity
Name
Sarah Phelan
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
ETD
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windows xp
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1.5
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2018-10-01T21:23:41
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2018-10-01T21:23:41
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Microsoft® Office Word 2007
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