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Co-selection of metal and antibiotic resistance in the gut microbial community of the Mummichog fish (Fundulus heteroclitus)

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TitleInfo
Title
Co-selection of metal and antibiotic resistance in the gut microbial community of the Mummichog fish (Fundulus heteroclitus)
Name (type = personal)
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Lloyd
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Nicole A.
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Nicole A. Lloyd
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Barkay
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Tamar
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Tamar Barkay
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Advisory Committee
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chair
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Fahrenfeld
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Nicole
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Nicole Fahrenfeld
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Advisory Committee
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internal member
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Zylstra
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Gerben
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Gerben Zylstra
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Advisory Committee
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internal member
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Nazaret
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Sylvie
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Sylvie Nazaret
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Advisory Committee
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outside member
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Rutgers University
Role
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degree grantor
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School of Graduate Studies
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Text
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theses
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DateCreated (qualifier = exact)
2018
DateOther (qualifier = exact); (type = degree)
2018-10
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2018
Place
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xx
Language
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eng
Abstract (type = abstract)
Microbial resistance to antibiotics is one of the most pressing health care concerns we are currently facing. Antibiotic resistance can be selected for by metals through the process known as co-selection. Co-selection occurs through two primary mechanisms: i) co-resistance, when genes encoding both are located together in the genome and ii), cross-resistance, when the same mechanism provides resistance to both (e.g. efflux pump proteins). This thesis describes co-selection in the gut microbiota of a forage feeder fish, the mummichog (Fundulus heteroclitus). By comparing gut microbiota from fish from a mercury contaminated site and a relatively clean site, we established the mummichog gut as a reservoir of antibiotic and mercury resistant microbiota. We further examined three representative isolates with interesting antibiotic and metal resistant profiles. Genome analyses revealed few instance of co-resistance, and instead, presented various chromosomally-encoded resistance genes, including some of public health concern, (i.e. a blaOXA carbapenemase), and contributions from cross-resistance. All three organisms contained a large number of efflux pump proteins, including multiple RND efflux pump operons. Testing of the strains shows that RND efflux pumps may be involved in resistance to several classes of drugs. A transcriptomic study is in progress to understand the regulation of arsenate resistance and the potential involvement of RND efflux pumps under metal stress. Together, this work describes the abundance and inventory of antibiotic resistance genes and mechanisms in the mummichog gut microbiome and establishes the fish gut as a reservoir of such genes. This work further adds to the understanding of the role of RND efflux pumps in resistance to antibiotics, which is critical in understanding multi-drug resistance in Gram negative bacteria. 
Subject (authority = RUETD)
Topic
Microbial Biology
Subject (authority = ETD-LCSH)
Topic
Drug resistance in microorganisms
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Rutgers University Electronic Theses and Dissertations
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ETD_9103
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electronic resource
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Note
Supplementary File: Appendix E
Extent
1 online resource (141 pages) : illustrations
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Nicole A. Lloyd
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier (type = doi)
doi:10.7282/t3-nqxf-q909
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Lloyd
GivenName
Nicole
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2018-07-16 11:49:00
AssociatedEntity
Name
Nicole Lloyd
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Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Type
Embargo
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2018-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2019-10-31
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 31st, 2019.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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