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From light to life

Descriptive

TitleInfo
Title
From light to life
SubTitle
the energy conversion and storage in plants and algae
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Yuan
NamePart (type = date)
1990-
DisplayForm
Yuan Zhang
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Dismukes
NamePart (type = given)
G. Charles
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G. Charles Dismukes
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Advisory Committee
Role
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chair
Name (type = personal)
NamePart (type = family)
Bhattacharya
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Debashish
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Debashish Bhattacharya
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Advisory Committee
Role
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internal member
Name (type = personal)
NamePart (type = family)
Boyd
NamePart (type = given)
Jeffrey M.
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Jeffrey M. Boyd
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Maliga
NamePart (type = given)
Pal
DisplayForm
Pal Maliga
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (qualifier = exact)
2018
DateOther (qualifier = exact); (type = degree)
2018-10
CopyrightDate (encoding = w3cdtf)
2018
Place
PlaceTerm (type = code)
xx
Language
LanguageTerm (authority = ISO639-2b); (type = code)
eng
Abstract (type = abstract)
Photosynthesis, the physico-chemical process converting sunlight into chemical energy, is the basis to feed the world and fuel the planet. To satisfy the growing demand for food and fuel, the efficiency of the natural photosynthesis needs to be optimized for maximum crop yield, while the photosynthetically assimilated carbon needs to be more sophisticatedly recruited for generating energy-dense renewable products. There are two objectives of this dissertation, the first is to explore the feasibility to boost biomass yield of crop plants by genetically engineering their photosystem II (PSII), and the second is to create robust microalgal transgenic strains with enhanced lipid content and CO2 utilization efficiency, which will contribute to microalgal biofuel production as well as CO2 mitigation.
In Chapter 2, we explore whether the prokaryotic design principal of PSII D1 subunit is applicable in a higher plant model Nicotiana tabacum. By introducing single point mutations into tobacco psbA gene (coding for the reaction center D1 subunit of Photosystem II) to mimic the cyanobacterial high-light and low-light D1 isoforms, the tobacco mutants exhibit the biophysical traits of the prokaryotic PSII. The tobacco mutant expressing the engineered high light isoform exhibits higher photosynthetic efficiency, higher tolerance to photoinhibition and increased biomass production under the tested light conditions. The only benefit of incorporating the cyanobacterial low light mutation into tobacco D1 protein is restricted to improving the Water Oxidizing Complex catalytic efficiency at low light intensity, while the biomass yield was impaired at all the tested light conditions.
In Chapter 3, Nannochloropsis oceanica CCMP1779 (N.o1779), the emerging oleaginous model alga, is chosen for application of the “push and pull” strategy to enhance its lipid productivity by metabolic engineering. The regulatory importance of citrate synthase (CIS) in directing carbon flux towards protein synthesis pathway, and the functional role of glycerol 3-phosphate dehydrogenase (G3PDH) in diverting carbon precursors from glycolysis to TAG assembly are fully examined in the transgenic strains of N.o1779. Downregulation of a putative endogenous gene encoding CIS via RNA interference technology and expression of a yeast gene encoding the cytosolic G3PDH lead to higher accumulation of the storage lipid triacylglycerols (TAGs) and increased abundance of the lipid building block free fatty acids, advancing our understanding of the genetic and molecular basis of algal TAG metabolism.
In Chapter 4, the goal was to create a robust industrial strain that can be cultivated in the open culture using flue gas as carbon source. By applying insertional mutagenesis combined with high-throughput screening strategy to the oleaginous microalga N.o1779, a winning mutant was successfully identified for its advantages in photoautotrophic growth and intrinsic photosynthetic efficiency under both normal growth condition and acidic environment. The genome sequencing project of this mutant currently in progress will potentially unlock the regulatory mechanism responsible for its beneficial phenotypes.
In summary, my dissertation advances the understanding of the PSII design principal and the central carbon metabolism in the oxygenic photosynthetic organisms. Novel genetic engineering strategies have also been developed throughout this dissertation to improve biomass productivity in a higher plant and enhance lipid productivity and carbon utilization in a eukaryotic microalga.
Subject (authority = RUETD)
Topic
Microbial Biology
Subject (authority = ETD-LCSH)
Topic
Biomass energy
Subject (authority = ETD-LCSH)
Topic
Microalgae
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_9312
PhysicalDescription
Form (authority = gmd)
electronic resource
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (128 pages : illustrations)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Yuan Zhang
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/t3-bkq3-ty29
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

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The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
ZHANG
GivenName
YUAN
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2018-10-03 10:44:37
AssociatedEntity
Name
YUAN ZHANG
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
Type
Embargo
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2018-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2020-10-30
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2020.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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2018-10-03T14:42:03
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2018-10-03T14:42:03
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