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Discovering the organohalide respiring capacity of Deltaproteobacteria in marine and estuarine environments
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LIU, JIE. Discovering the organohalide respiring capacity of Deltaproteobacteria in marine and estuarine environments. Retrieved from https://doi.org/doi:10.7282/t3-1qkp-re44

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TitleDiscovering the organohalide respiring capacity of Deltaproteobacteria in marine and estuarine environments
NameLIU, JIE (author); Häggblom, Max M (chair); Kerkhof, Lee (internal member); Vetriani, Costantino (internal member); Fennell, Donna (outside member); Rutgers University; School of Graduate Studies
Date Created2019
Other Date2019-01 (degree)
SubjectMicrobial Biology, Biodegradation, Bacteria--Morphology
Extent1 online resource (216 pages) : illustrations
DescriptionOrganohalides are widespread in the environment from both anthropogenic and natural sources. The marine environment is a major reservoir of organohalides, particularly organobromides. Organohalide respiration is the process mediated by bacteria that utilize organohalides as electron acceptors for energy conservation. This process is of importance in the degradation organohalide pollutants and the overall organohalide cycle. The objective of this study was to investigate the ecophysiology of organohalide respiring bacteria (OHRBs) with a focus on examining their utilization of organobromine compounds, evaluating their substrate range and activity under different conditions, and determining their reductive dehalogenase gene diversity and gene expression.
Investigation of Desulfoluna spongiiphila, a marine sponge associated organobromide-respiring bacterium, revealed that the expression of one reductive dehalogenase gene was significantly upregulated in response to bromophenol and sponge extracts. The transcriptomic and proteomic analysis further confirmed the function of this reductive dehalogenase gene in reductive dehalogenation of bromophenol. Two bacterial strains with organobromide respiring ability were isolated from New Jersey estuarine sediments, which are representatives of a novel Halodesulfovibrio species. Three reductive dehalogenase genes are present in their genomes, one of which showed significant upregulation in gene expression in response to bromophenols. In addition, the overall organohalide respiring capacity of members of the Class Deltaproteobacteria was evaluated by surveying the genome database for the prevalence of reductive dehalogenase genes in their genomes. Result showed that approximately 10% of Deltaproteobacteria contain reductive dehalogenase genes in their genomes, which is an indicator for potential organohalide respiring ability. Three of these strains were experimentally confirmed to have organobromide respiring ability. In total, six organobromide respiring Deltaproteobacteria, all marine or estuarine origin, were investigated in this study. One common reductive dehalogenase gene cluster (namely brp gene) was present in all the genomes and the expression of brpA gene was significantly upregulated in the presence of bromophenols in all the strains. This study highlights the potential role of Deltaproteobacteria in the marine organohalide cycle.
NotePh.D.
NoteIncludes bibliographical references
Noteby Jie Liu
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/t3-1qkp-re44
Languageeng
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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