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Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus

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TypeOfResource
Text
TitleInfo
Title
Differential modes of crosslinking establish spatially distinct regions of peptidoglycan in Caulobacter crescentus
TitleInfo (type = alternative)
Title
Distinct domains of peptidoglycan in C. crescentus
Name (type = personal)
NamePart (type = family)
Stankeviciute
NamePart (type = given)
Gabriele
Affiliation
Center for Computational and Integrative Biology (CCIB), Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Miguel
NamePart (type = given)
Amanda V.
Affiliation
Stanford University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Radkov
NamePart (type = given)
Atanas
Affiliation
University of California, San Francisco
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal)
NamePart (type = family)
Chou
NamePart (type = given)
Seemay
Role
RoleTerm (type = text); (authority = marcrt)
author
Affiliation
University of California, San Francisco
Name (type = personal)
NamePart (type = family)
Huang
NamePart (type = given)
Kerwyn Casey
Affiliation
Stanford University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = personal); (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (valueURI = http://orcid.org/0000-0003-3941-6233)
NamePart (type = family)
Klein
NamePart (type = given)
Eric A.
Affiliation
Biology, Rutgers University
Role
RoleTerm (type = text); (authority = marcrt)
author
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Biology
Name (type = corporate); (authority = RutgersOrg-School)
NamePart
Camden College of Arts and Sciences
Name (type = corporate); (authority = RutgersOrg-Department)
NamePart
Center for Computational and Integrative Biology (CCIB)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact); (keyDate = yes)
2019
PhysicalDescription
InternetMediaType
application/pdf
Extent
1 online resource (48 pages) : illustrations
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Subject (authority = local)
Topic
Transpeptidase
Subject (authority = local)
Topic
Stalk biosynthesis
Subject (authority = local)
Topic
LD crosslinks
Subject (authority = local)
Topic
LD transpeptidases
Subject (authority = local)
Topic
Lysozyme
Subject (authority = local)
Topic
Cell shape
Abstract (type = summary)
The diversity of cell shapes across the bacterial kingdom reflects evolutionary pressures that have produced physiologically important morphologies. While efforts have been made to understand the regulation of some prototypical cell morphologies such as that of rod-shaped Escherichia coli, little is known about most cell shapes. For Caulobacter crescentus, polar stalk synthesis is tied to its dimorphic life cycle, and stalk elongation is regulated by phosphate availability. Based on the previous observation that C. crescentus stalks are lysozyme-resistant, we compared the composition of the peptidoglycan cell wall of stalks and cell bodies and identified key differences in peptidoglycan crosslinking. Cell-body peptidoglycan contained primarily DD-crosslinks between meso-diaminopimelic acid and D-alanine residues, whereas stalk peptidoglycan had more LD-transpeptidation (meso-diaminopimelic acid-meso-diaminopimelic acid), mediated by LdtD. We determined that ldtD is dispensable for stalk elongation; rather, stalk LD-transpeptidation reflects an aging process associated with low peptidoglycan turnover in the stalk. We also found that lysozyme resistance is a structural consequence of LD-crosslinking. Despite no obvious selection pressure for LD-crosslinking or lysozyme resistance in C. crescentus, the correlation between these two properties was maintained in other organisms, suggesting that DAP-DAP crosslinking may be a general mechanism for regulating bacterial sensitivity to lysozyme.
Extension
DescriptiveEvent
Type
Citation
DateTime (encoding = w3cdtf); (qualifier = exact)
2019-02-03
AssociatedObject
Type
Journal
Relationship
Has part
Name
Molecular Microbiology
Identifier (type = volume and issue)
111(4)
Reference (type = url)
https://doi.org/10.1111/mmi.14199
Detail
995-1008
RelatedItem (type = host)
TitleInfo
Title
Klein, Eric A.
Identifier (type = local)
rucore30196400001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-geqz-g349
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RightsDeclaration (AUTHORITY = FS); (TYPE = [FS] statement #1); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = start); (encoding = w3cdtf); (qualifier = exact)
2019-02-03
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after February 03, 2020.
DateTime (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-02-03
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RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.6
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2019-03-28T17:38:53
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2019-03-28T17:38:55
ApplicationName
Acrobat Distiller 10.1.7 (Windows)
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