Home
Resource
Process of creating a more efficient real-time quantitative PCR assay to detect Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, and Borrelia miyamotoi that inhabit deer ticks
PDF
PDF format is widely accepted and good for printing.
Plug-in required
PDF-1(4.51 MB)
Citation & Export
View Usage Statistics
Staff View

Citation & Export
Hide

Simple citation

Allan, Rachael. Process of creating a more efficient real-time quantitative PCR assay to detect Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, and Borrelia miyamotoi that inhabit deer ticks. Retrieved from https://doi.org/doi:10.7282/t3-t6j6-fn98

Export

Click here for information about Citation Management Tools at Rutgers.

Statistics
Hide

Description

TitleProcess of creating a more efficient real-time quantitative PCR assay to detect Anaplasma phagocytophilum, Babesia microti, Borrelia burgdorferi, and Borrelia miyamotoi that inhabit deer ticks
NameAllan, Rachael (author); Toledo, Alvaro (chair); Zylstra, Gerben (internal member); Kerkhof, Lee (internal member); Rutgers University; School of Graduate Studies
Date Created2019
Other Date2019-05 (degree)
SubjectMicrobial Biology, Tick-borne diseases -- Testing, Anaplasmosis -- Testing, Babesiosis -- Testing, Borrelia burgdorferi -- Testing, Borrelia -- Testing
Extent1 online resource (xviii, 108 pages) : illustrations
DescriptionIxodes scapularis, the most abundant tick species in New Jersey, is a vector for a host of human diseases, including Lyme disease, Anaplasmosis, Babesiosis, and B. miyamotoi. The prevalence of these diseases continues to steadily increase, and it has become critical to develop molecular methods to detect them. This project focuses on transitioning from a standard PCR and gel electrophoresis methodology to real-time PCR to screen for four common pathogens found in deer ticks – B. burgdorferi, B. miyamotoi, A. phagocytophilum, and B. microti. Primers and probes were created to target genes that were unique to each pathogen. They were tested using standard PCR and gel electrophoresis and were then optimized with real-time PCR using SYBR Green and TaqMan methodologies. The primer specificity was also assessed using tick DNA as a template utilizing a SYBR Green methodology. 158 deer ticks were tested for the presence of pathogens – 11.39% of deer ticks tested positive for B. burgdorferi, 6.33% tested positive for B. microti, 6.96% tested positive for B. miyamotoi, and 10.76% tested positive for A. phagocytophilum. With all of these steps completed, a multiplex PCR assay can be created to screen for all four pathogens in one single test tube.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-t6j6-fn98
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
Version 8.5.5
Rutgers University Libraries - Copyright ©2024