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Effects of volatile 1-octen-3-ol and biocontrol strain genetics on mycotoxin accumulation

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TitleInfo
Title
Effects of volatile 1-octen-3-ol and biocontrol strain genetics on mycotoxin accumulation
Name (type = personal)
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Pennerman
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Kayla K.
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Kayla K. Pennerman
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author
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Bennett
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Joan W
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Joan W Bennett
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Advisory Committee
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chair
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Zhang
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Ning
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Ning Zhang
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Advisory Committee
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internal member
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White
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James F
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James F White
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Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
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Kahn
NamePart (type = given)
Peter
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Peter Kahn
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Advisory Committee
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outside member
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Rutgers University
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degree grantor
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School of Graduate Studies
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school
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theses
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2019
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2019-05
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2019
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English
Abstract (type = abstract)
Mycotoxins are fungal secondary metabolites that contaminate global food supplies by infecting pre- and post-harvest crops. Contaminated foods are highly hazardous to human health and cause economic losses to agricultural and food industries. Control of mycotoxin accumulation generally involves prevention such as use of chemical and biological agents to inhibit growth of toxigenic fungal strains. Two well-known toxins, patulin and aflatoxin, are among the most harmful compounds produced respectively by the fungi Penicillium expansum and Aspergillus flavus. Using molecular and computational methods, I investigated the biological mechanisms by which volatile 1-octen-3-ol affects patulin production by P. expansum and how genetic differences among non-aflatoxigenic A. flavus strains affect biocontrol effectiveness. My results demonstrate that exogenous volatile 1-octen-3-ol increases patulin production on a medium that normally suppresses biosynthesis of the mycotoxin. This increase correlates with an increased expression of a glucose oxidase gene and differential expression of thirty other genes involved in membrane transport, oxidation-reduction and carbohydrate metabolism. I compared the genomes and transcriptomes of two non-aflatoxigenic strains that are good or poor at biocontrol against aflatoxins to other sequenced A. flavus strains. The former contains an enriched number of genes predicted to be involved in oxidation-reduction processes that were not found in other inspected A. flavus strains. The non-aflatoxigenic strain that is a poor biocontrol agent has increased relative expression of six genes involved in stress response, which may help explain why the strain grows more slowly than other tested strains. A common theme that emerged from my studies on the genomes and transcriptomes of P. expansum and A. flavus was the identification of putative oxidation-reduction genes, indicating an important role of redox reactions in mycotoxin production. Overall, I obtained evidence for how 1-octen-3-ol induces patulin production and how high expression of stress response genes might impede competitive growth for aflatoxin biocontrol. It is my hope that this research will contribute to our general understanding of how volatile signaling and gene expression influence mycotoxin production and accumulation.
Subject (authority = RUETD)
Topic
Plant Biology
Subject (authority = LCSH)
Topic
Aspergillus flavus
Subject (authority = LCSH)
Topic
Mycotoxins -- Genetic aspects
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Rutgers University Electronic Theses and Dissertations
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ETD_9711
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1 online resource (x, 262 pages) : illustrations
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier (type = doi)
doi:10.7282/t3-xrgn-d904
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

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The author owns the copyright to this work.
RightsHolder (type = personal)
Name
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Pennerman
GivenName
Kayla
MiddleName
K.
Role
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Type
Permission or license
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2019-04-08 08:21:41
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Kayla Pennerman
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Affiliation
Rutgers University. School of Graduate Studies
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Type
Embargo
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2019-05-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2020-05-30
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after May 30th, 2020.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2019-04-08T08:18:19
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