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Development of a click chemistry based screening methodology for the directed evolution of glycosynthases

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TitleInfo
Title
Development of a click chemistry based screening methodology for the directed evolution of glycosynthases
Name (type = personal)
NamePart (type = family)
Agrawal
NamePart (type = given)
Ayushi
NamePart (type = date)
1993-
DisplayForm
Ayushi Agrawal
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Chundawat
NamePart (type = given)
Shishir P.S
DisplayForm
Shishir P.S Chundawat
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Haoran
DisplayForm
Haoran Zhang
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Gormley
NamePart (type = given)
Adam J.
DisplayForm
Adam J. Gormley
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (qualifier = exact)
2019
DateOther (encoding = w3cdtf); (qualifier = exact); (type = degree)
2019-10
CopyrightDate (encoding = w3cdtf); (qualifier = exact)
2019
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract (type = abstract)
Glycans are the most abundant biomolecules in nature and play a significant role in biological systems. They are involved in diverse biological processes such as intercellular signaling, intracellular metabolism, and protein folding. Hence, while the importance of glycans in the fields of chemistry, biology, and bioengineering is being increasingly recognized, limited progress has been made due to the lack of well-defined glycan based reagents available for research applications. Development of efficient chemoenzymatic routes for synthesis of the designer carbohydrates (or glycans) has therefore gained momentum in recent years. Glycosynthases are mutant glycosidase enzymes that can catalyze the formation of glycosidic bonds (between monosaccharide groups) instead of breaking/cleaving these bonds. But identifying a suitable glycosidase from a library of mutants that can act as an efficient glycosynthase for targeted donor or acceptor sugars is still very challenging. This study focuses on the development of a high-throughput screening (HTS) method to rapidly screen and evolve glycosynthase variants generated using traditional directed evolution techniques to enhance catalytic activity or product specificity. A model glycosynthase was used here for validation of this HTS method. Random mutagenesis (via error-prone PCR) was performed on this model glycosynthase to generate a library of mutants that were then screened using this validated HTS method.
Subject (authority = RUETD)
Topic
Chemical and Biochemical Engineering
Subject (authority = local)
Topic
Click chemistry
Subject (authority = LCSH)
Topic
Glycosidases
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_10163
PhysicalDescription
Form (authority = gmd)
InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (xi, 102 pages) : illustrations
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/t3-ax6t-5f73
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Agrawal
GivenName
Ayushi
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2019-08-12 14:57:04
AssociatedEntity
Name
Ayushi Agrawal
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
RightsEvent
Type
Embargo
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2019-10-31
DateTime (encoding = w3cdtf); (qualifier = exact); (point = end)
2021-10-30
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2021.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

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ETD
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windows xp
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2019-08-15T15:21:15
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2019-08-15T15:21:15
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Microsoft® Word 2016
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