LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract (type = abstract)
Traumatic brain injury (TBI) is a leading cause of disability and death in the United States and worldwide. Endogenous neural stem/progenitor cells (NSPCs) in the adult are a potential source for injury recovery. However, much about the response of injury-activated NSPCs is still unknown. Notch signaling is critical for maintaining NSPC status during embryonic development and transiently activated after injury. In the first part of this thesis, the role of Notch signaling after TBI is investigated using a Notch1CR2-GFP transgenic mouse model. During development, GFP mainly marks interneuron progenitor cells. A closed head injury (CHI) in this transgenic mouse was performed to determine the response of injury-activated NSPCs. CHI induces neuroinflammation, cell death, and the expression of typical TBI markers, validating the animal model. In addition, CHI induces cell proliferation in GFP+ cells expressing NSPC markers, e.g., Notch1 and Nestin. A significant higher percentage of GFP+ GABAergic interneurons was observed in the CHI brain, with no significant change in oligodendrocyte lineage between the CHI and sham animal groups. Since injury is known to activate astrogliosis, these results suggest that injury-induced GFP+ NSPCs preferentially differentiate into GABAergic neurons. Our study establishes that Notch1CR2-GFP transgenic mouse is a useful tool for the study of NSPC behavior in vivo after TBI.
In the second part of this thesis, the role of Gsx1, a neurogenic transcription factor, on promoting Notch1 expression and neurogenesis is investigated. A lentivirus system is used to deliver Gsx1 at the injury site after closed head injury (CHI) in the Notch1CR2-GFP transgenic mice. We identify that CHI increases GFP+ cell, during the acute phase of TBI and increasingly label neurons during the chronic phase of TBI. Lentivirus-mediated Gsx1 overexpression increases Notch1 expressing cells in the cerebral cortex and hippocampus; these virally transduced cells proliferate and mark NSPCs during the subacute phase of TBI and primarily label glutamatergic neurons during the chronic phase of TBI. The role of Gsx1 promoting Notch signaling and neurogenesis after TBI represents a new therapeutic for the treatment of TBI. Unveiling the potential of NSPCs to TBI (e.g., proliferation and differentiation) will identify new therapeutic strategy for the treatment of brain trauma.
Subject (authority = RUETD)
Topic
Biomedical Engineering
Subject (authority = LCSH)
Topic
Neural stem cells
Subject (authority = LCSH)
Topic
Transgenic mice
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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