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Neural stem cell transplantation to treat lumbosacral spinal cord injury
Descriptive
TitleInfo
Title
Neural stem cell transplantation to treat lumbosacral spinal cord injury
Name
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NamePart
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Chiang
NamePart
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Ning
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Ning Chiang
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author
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Young
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Wise Young
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chair
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Shors
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Tracey
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Tracey Shors
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Advisory Committee
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internal member
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(type = family)
Cai
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Qian
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Qian Cai
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Advisory Committee
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(authority = RULIB)
internal member
Name
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(type = family)
Dezawa
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Mari
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Mari Dezawa
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Advisory Committee
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outside member
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Rutgers University
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degree grantor
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School of Graduate Studies
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Text
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theses
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2019
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2019-10
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2019
Language
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English
Abstract
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A third of human spinal cord injuries (SCIs) involve the lumbosacral spinal cord (LSC), but few studies are on LSC injury (LSCI) and no functional treatments are available to date. Unlike cervical or thoracic SCI that damages primarily the fiber tracts, LSCI disrupts both fiber tracts and neurons comprising the motor centers for walking, bladder, bowel and sexual functions. Thus, treatments for LSCI need not only axonal regeneration but also neuronal replacement. The ideal therapy is to transplant cells that have neuronal potential that can replace the damaged or lost neurons after LSCI. Initially, I cultured neural stem cells (NSCs) isolated from the subventricular zone (SVZ) of neonatal Fischer 344 rats and other cell types as the sources for transplantation. However, those cells either remained as NSCs or produced astrocytes instead of neurons after transplantation. Neither lithium treatment nor silencing of the phosphatase and tensin homolog (PTEN) gene increased the percentage of neurons in culture. Silencing PTEN reduced neurons and produced more oligodendrocytes. Therefore, I transplanted human induced-pluripotent stem cell-derived neural stem cells (iPSC-NSCs) into immune-compromised Rowett nude (RNU) rats after LSCI. These human iPSC-NSCs survived, migrated and grew long processes 10 weeks after transplantation, showing the potential of human iPSC-NSCs as a future therapy for LSCI. However, the transplants did not improve functional recovery, and the reasons why will be discussed.
Subject
(authority = RUETD)
Topic
Neuroscience
Subject
(authority = LCSH)
Topic
Neural stem cells -- Transplantation
Subject
(authority = LCSH)
Topic
Lumbar vertebrae -- Wounds and injuries -- Treatment
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Rutgers University Electronic Theses and Dissertations
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ETD_10220
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text/xml
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1 online resource (xi, 93 pages) : illustrations
Note
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Ph.D.
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Includes bibliographical references
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier
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doi:10.7282/t3-qamz-bv21
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Chiang
GivenName
Ning
Role
Copyright Holder
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Type
Permission or license
DateTime
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2019-09-23 10:51:09
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Name
Ning Chiang
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Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Embargo
DateTime
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2019-10-31
DateTime
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2021-10-30
Detail
Access to this PDF has been restricted at the author's request. It will be publicly available after October 30th, 2021.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2019-09-23T15:05:46
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2019-09-23T15:05:46
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