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Arctic tundra soil bacterial communities active at subzero temperatures detected by stable isotope probing

Descriptive

TypeOfResource
Text
TitleInfo
Title
Arctic tundra soil bacterial communities active at subzero temperatures detected by stable isotope probing
Name (type = personal)
NamePart (type = family)
Gadkari
NamePart (type = given)
Preshita S.
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Biochemistry and Microbiology, Rutgers University
Name (type = personal)
NamePart (type = family)
McGuinness
NamePart (type = given)
Lora R.
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
Marine and Coastal Sciences, Rutgers University
Name (type = personal)
NamePart (type = family)
Männistö
NamePart (type = given)
Minna K.
Affiliation
Natural Resources Institute Finland
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Kerkhof
NamePart (type = given)
Lee J.
Affiliation
Marine and Coastal Sciences, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = orcid); (authorityURI = http://id.loc.gov/vocabulary/identifiers/orcid.html); (type = personal); (valueURI = http://orcid.org/0000-0001-6307-7863)
NamePart (type = family)
Häggblom
NamePart (type = given)
Max M.
Affiliation
Biochemistry and Microbiology, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Biochemistry and Microbiology
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Marine and Coastal Sciences
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Environmental and Biological Sciences (SEBS)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Note (type = peerReview)
Peer reviewed
OriginInfo
DateCreated (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2019
Abstract (type = Abstract)
Arctic soils store vast amounts of carbon and are subject to intense climate change. While effects of thaw on the composition and activities of Arctic tundra microorganisms has been examined extensively, little is known about the consequences of temperature fluctuations within the subzero range in seasonally frozen or permafrost soils. This study identified tundra soil bacteria active at subzero temperatures using stable isotope probing (SIP). Soils from Kilpisjärvi, Finland were amended with 13C-cellobiose and incubated at 0, -4, and -16°C for up to 40 weeks. 16S rRNA gene sequence analysis of 13C-labelled DNA revealed distinct subzero-active bacterial taxa. The SIP experiments demonstrated that diverse bacteria, including members of Candidatus Saccharibacteria, Melioribacteraceae, Verrucomicrobiaceae, Burkholderiaceae, Acetobacteraceae, Armatimonadaceae, and Planctomycetaceae were capable of synthesizing 13C-DNA at subzero temperatures. Differences in subzero temperature optima were observed, for example with members of Oxalobacteraceae and Rhizobiaceae found to be more active at 0°C than at -4°C or -16°C, whereas Melioribacteriaceae were active at all subzero temperatures tested. Phylogeny of 13C-labelled 16S rRNA genes from the Melioribacteriaceae, Verrucomicrobiaceae, and Candidatus Saccharibacteria suggested that these taxa formed subzero-active clusters closely related to members from other cryo-environments. This study demonstrates that subzero temperatures impact active bacterial community composition and activity which may influence biogeochemical cycles.
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
1 online resource (34 pages) : illustration
Extension
DescriptiveEvent
Type
Citation
AssociatedObject
Name
FEMS Microbiology Ecology
Type
Journal
Relationship
Has part
Reference (type = url)
https://doi.org/10.1093/femsec/fiz192
RelatedItem (type = host)
TitleInfo
Title
Häggblom, Max M.
Identifier (type = local)
rucore30202400001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-9xqb-6e24
Genre (authority = ExL-Esploro)
Accepted Manuscript
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Rights

RightsDeclaration (AUTHORITY = FS); (TYPE = [FS] statement #1); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = start); (encoding = w3cdtf); (qualifier = exact)
2019-11-28
DateTime (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-11-28
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after November 28, 2020.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
Version
1.5
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2019-11-29T14:12:59
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2019-11-29T14:13:37
ApplicationName
Microsoft® Word 2016
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