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Grafting of aspirin on to gelatin for anti-inflammatory effect in scaffold development
Descriptive
TitleInfo
Title
Grafting of aspirin on to gelatin for anti-inflammatory effect in scaffold development
Name
(type = personal)
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(type = family)
Moghe
NamePart
(type = given)
Mihir Ravindra
NamePart
(type = date)
1993-
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Mihir Ravindra Moghe
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(authority = RULIB)
author
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(type = personal)
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Shreiber
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David I.
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David I. Shreiber
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Advisory Committee
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chair
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Roth
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Charles M.
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Charles M. Roth
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Advisory Committee
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internal member
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Gormley
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Adam J.
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Adam J. Gormley
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Advisory Committee
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internal member
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Rutgers University
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degree grantor
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School of Graduate Studies
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school
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Text
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theses
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2019
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2019-10
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2019
Language
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English
Abstract
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In recent times, there is an increasing interest in the field of tissue engineering for biochemical or biomedical use. A core component of tissue engineering is the scaffolds used for controlling cell growth. These scaffolds are made of a plethora of materials, each with their own distinct properties, which can be further modified by the addition of secondary substances like drug molecules.
Gelatin is a commonly used biomaterial for the formation of gel scaffolds. However, due to its water solubility, there have been issues trying to graft water-insoluble materials on to it. In this work, we try to graft aspirin, a water-insoluble drug molecule, to gelatin and characterize the properties of the resultant product.
The grafting of the gelatin and aspirin was performed through amidation with dicyclocarbodiimide (DCC) and N-hydroxysuccinimide (NHS) as intermediate coupling agent. During this process, experiments were conducted to find appropriate solvents for a homogenous reaction. The resultant product was purified utilizing multiple filtration passes, dialysis, and lyophilization.
After production of the aspirin-grafted-gelatin (AGG), it was characterized and tested for its gelling and anti-inflammatory effect. The below-mentioned tests were conducted to characterize the product and confirm the expected results.
Free amine detection using fluorescamine assay was conducted to detect the presence of a secondary material which may have replaced the free amines in gelatin. It was assumed that these amines were consumed in amidation with aspirin. FTIR spectroscopy was used to determine the characteristics of the materials within the AGG. This was performed by comparing the troughs of AGG wave numbers with the troughs of gelatin and aspirin at the same wave numbers. To determine the gel scaffolding capability of AGG, gelling test of AGG was conducted. Finally, to test whether AGG has retained aspirin’s anti-inflammatory properties, cell testing using RAW 264.7 macrophages was conducted. Enzymatic breakdown test using trypsin was also run on AGG to calculate its breakdown rate as compared to gelatin.
Gelatin is a commonly used biomaterial for the formation of gel scaffolds. However, due to its water solubility, there have been issues trying to graft water-insoluble materials on to it. In this work, we try to graft aspirin, a water-insoluble drug molecule, to gelatin and characterize the properties of the resultant product.
The grafting of the gelatin and aspirin was performed through amidation with dicyclocarbodiimide (DCC) and N-hydroxysuccinimide (NHS) as intermediate coupling agent. During this process, experiments were conducted to find appropriate solvents for a homogenous reaction. The resultant product was purified utilizing multiple filtration passes, dialysis, and lyophilization.
After production of the aspirin-grafted-gelatin (AGG), it was characterized and tested for its gelling and anti-inflammatory effect. The below-mentioned tests were conducted to characterize the product and confirm the expected results.
Free amine detection using fluorescamine assay was conducted to detect the presence of a secondary material which may have replaced the free amines in gelatin. It was assumed that these amines were consumed in amidation with aspirin. FTIR spectroscopy was used to determine the characteristics of the materials within the AGG. This was performed by comparing the troughs of AGG wave numbers with the troughs of gelatin and aspirin at the same wave numbers. To determine the gel scaffolding capability of AGG, gelling test of AGG was conducted. Finally, to test whether AGG has retained aspirin’s anti-inflammatory properties, cell testing using RAW 264.7 macrophages was conducted. Enzymatic breakdown test using trypsin was also run on AGG to calculate its breakdown rate as compared to gelatin.
Subject
(authority = RUETD)
Topic
Chemical and Biochemical Engineering
Subject
(authority = LCSH)
Topic
Tissue scaffolds
Subject
(authority = LCSH)
Topic
Gelatin
Subject
(authority = LCSH)
Topic
Aspirin
RelatedItem
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
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ETD_10236
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text/xml
Extent
1 online resource (ix, 37 pages) : illustrations
Note
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M.S.
Note
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Includes bibliographical references
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/t3-y8v1-em26
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
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Moghe
GivenName
Mihir
Role
Copyright Holder
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Type
Permission or license
DateTime
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2019-09-11 21:31:43
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Name
Mihir Moghe
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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