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The first characterization of the WASH complex in C. elegans endocytic recycling

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TitleInfo
Title
The first characterization of the WASH complex in C. elegans endocytic recycling
Name (type = personal)
NamePart (type = family)
Smolyn
NamePart (type = given)
Jennifer
NamePart (type = date)
1992-
DisplayForm
Jennifer Smolyn
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Soto
NamePart (type = given)
Martha
DisplayForm
Martha Soto
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Grant
NamePart (type = given)
Barth
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Barth Grant
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = personal)
NamePart (type = family)
Wadsworth
NamePart (type = given)
William
DisplayForm
William Wadsworth
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
co-chair
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
OriginInfo
DateCreated (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2020
DateOther (encoding = w3cdtf); (qualifier = exact); (type = degree)
2020-01
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract (type = abstract)
The quantity and distribution of proteins in the plasma membrane play an essential role in regulating a cell’s response to its environment and resulting physiology. This cell surface protein mosaic is largely influenced by the endocytic recycling pathway, misregulation of which has been implicated in a variety of human conditions, including neurodegenerative diseases and cancer. Endocytosis and subsequent intracellular movement of cargo requires both membrane fission and vesicle movement, with the force for these processes provided by a spatially and temporally regulated dynamic branched actin network, generated by Arp2/3-mediated actin polymerization. While it has been determined that endocytosis requires the nucleation promoting factors WASP and WAVE at the plasma membrane, a role for the structurally-related WASH complex for this process in C. elegans remains more elusive. In mammalian systems, the WASH complex seems to act at early endosomes, facilitating retrograde trafficking via its FAM21 subunit interacting with retromer. However, a homologous protein to FAM21 in the Caenorhabditis elegans WASH complex has yet to be identified. This work provides the first characterization of the C. elegans WASH complex, detailing its function on RAB-5-, PI(3)P-positive early endosomes to ensure proper sorting during endocytic recycling, likely through retrograde trafficking to the Golgi apparatus. Through RNAis and genetic crosses, these experiments reveal a functionally important role for the WASH complex in this pathway that is distinct from other nucleation promoting factors, including the WAVE complex, despite similarities between the two. Furthermore, proper recycling may be mediated in part by the protein C05G5.2, as these data suggest this could be the previously unidentified C. elegans FAM21 homolog.
Subject (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (authority = local)
Topic
WASH
Subject (authority = LCSH)
Topic
Caenorhabditis elegans
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_10509
PhysicalDescription
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InternetMediaType
application/pdf
InternetMediaType
text/xml
Extent
1 online resource (viii, 69 pages) : illustrations
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-sgsb-mr58
Genre (authority = ExL-Esploro)
ETD graduate
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Smolyn
GivenName
Jennifer
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2020-01-07 19:38:45
AssociatedEntity
Name
Jennifer Smolyn
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
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ETD
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windows xp
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1.4
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DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-01-08T16:18:30
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-01-08T16:18:30
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