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Constructing E. coli co-cultures for de novo biosynthesis of natural product acacetin

Descriptive

TypeOfResource
Text
TitleInfo
Title
Constructing E. coli co-cultures for de novo biosynthesis of natural product acacetin
Name (type = personal)
NamePart (type = family)
Wang
NamePart (type = given)
Xiaonan
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Shao
NamePart (type = given)
Alan
Role
RoleTerm (authority = marcrt); (type = text)
author
Affiliation
School of Arts and Sciences (SAS) (New Brunswick), Rutgers University
Name (type = personal)
NamePart (type = family)
Li
NamePart (type = given)
Zhenghong
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Policarpio
NamePart (type = given)
Lizelle
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (type = personal)
NamePart (type = family)
Zhang
NamePart (type = given)
Haoran
Affiliation
Chemical & Biochemical Engineering, Rutgers University
Role
RoleTerm (authority = marcrt); (type = text)
author
Name (authority = RutgersOrg-Department); (type = corporate)
NamePart
Chemical & Biochemical Engineering
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Engineering
Name (authority = RutgersOrg-School); (type = corporate)
NamePart
School of Arts and Sciences (SAS) (New Brunswick)
Genre (authority = RULIB-FS)
Article, Refereed
Genre (authority = NISO JAV)
Accepted Manuscript (AM)
Genre (authority = ExL-Esploro)
Accepted manuscript
Note (type = peerReview)
Peer reviewed
OriginInfo
DateCreated (encoding = w3cdtf); (keyDate = yes); (qualifier = exact)
2020
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
PhysicalDescription
InternetMediaType
application/pdf
Extent
1 online resource (22 pages) : illustrations
Extension
DescriptiveEvent
Type
Citation
AssociatedObject
Name
Biotechnology Journal
Type
Journal
Relationship
Has part
Reference (type = url)
https://doi.org/10.1002/biot.202000131
Subject (authority = local)
Topic
Modular co-culture engineering
Subject (authority = local)
Topic
E. coli
Subject (authority = local)
Topic
Acacetin
Subject (authority = local)
Topic
Pathway balancing
Subject (authority = local)
Topic
Heterologous biosynthesis
Abstract (type = abstract)
Modular co-culture engineering is an emerging approach for biosynthesis of complex natural products. In this study, we constructed microbial co-cultures composed of two and three E. coli strains, respectively, for de novo biosynthesis of flavonoid acacetin, a value-added natural compound possessing numerous demonstrated biological activities, from simple carbon substrate glucose. To this end, the heterologous biosynthetic pathway was divided into different modules, each of which was accommodated in a dedicated E. coli strain for functional expression. After the optimization of the inoculation ratio between the constituent strains, the engineered cocultures showed a 4.83-fold improvement in production comparing to the mono-culture controls. Importantly, cultivation of the three-strain co-culture in shake flasks resulted in the production of 20.3 mg/L acacetin after 48 h. To our knowledge, this is the first report on acacetin de novo biosynthesis in a heterologous microbial host. The results of this work confirm the effectiveness of modular co-culture engineering for complex flavonoid biosynthesis.
RelatedItem (type = host)
TitleInfo
Title
Zhang, Haoran
Identifier (type = local)
rucore30193200001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-6zb2-vt86
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RightsDeclaration (AUTHORITY = FS); (TYPE = [FS] statement #1); (ID = rulibRdec0004)
Copyright for scholarly resources published in RUcore is retained by the copyright holder. By virtue of its appearance in this open access medium, you are free to use this resource, with proper attribution, in educational and other non-commercial settings. Other uses, such as reproduction or republication, may require the permission of the copyright holder.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsEvent
Type
Permission or license
AssociatedObject
Type
License
Name
Multiple author license v. 1
Detail
I hereby grant to Rutgers, The State University of New Jersey (Rutgers) the non-exclusive right to retain, reproduce, and distribute the deposited work (Work) in whole or in part, in and from its electronic format, without fee. This agreement does not represent a transfer of copyright to Rutgers.Rutgers may make and keep more than one copy of the Work for purposes of security, backup, preservation, and access and may migrate the Work to any medium or format for the purpose of preservation and access in the future. Rutgers will not make any alteration, other than as allowed by this agreement, to the Work.I represent and warrant to Rutgers that the Work is my original work. I also represent that the Work does not, to the best of my knowledge, infringe or violate any rights of others.I further represent and warrant that I have obtained all necessary rights to permit Rutgers to reproduce and distribute the Work and that any third-party owned content is clearly identified and acknowledged within the Work.By granting this license, I acknowledge that I have read and agreed to the terms of this agreement and all related RUcore and Rutgers policies.
RightsEvent
Type
Embargo
DateTime (point = start); (encoding = w3cdtf); (qualifier = exact)
2020-06-23
DateTime (point = end); (encoding = w3cdtf); (qualifier = exact)
2021-06-23
Detail
Access to this PDF has been restricted at the publisher's request. It will be publicly available after June 23, 2021.
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Technical

RULTechMD (ID = TECHNICAL1)
ContentModel
Document
CreatingApplication
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-07-04T04:53:00
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2020-07-04T04:53:00
ApplicationName
Microsoft Office Word
Version
16.0000
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