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Examining spirodela small rnas & the world's smallest flowers

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Examining spirodela small rnas & the world's smallest flowers
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Fourounjian
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Paul
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1990
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Fourounjian, Paul, 1990-
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author
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Dismukes
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Gerard Charles
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Gerard Charles Dismukes
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chair
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James
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James White
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internal member
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Belanger
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Faith C.
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Faith C. Belanger
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Slovin
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Janet P.
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Janet P. Slovin
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Advisory Committee
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Rutgers University
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degree grantor
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School of Graduate Studies
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theses
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2020
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2020-05
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English
Abstract (type = abstract)
When I joined the duckweed community the interest was largely on sustainably providing clean water, food, and fuel as reflected in the Introduction and Conclusion. Then a fellow student of Dr. Messing, Dr. Wang, published the Spirodela polyrhiza 7498 genome and transcriptome. My thesis project would complement these with the Spirodela miRNA and target catalog, and investigate the relationship of the miRNAs 156 and 172 to the neotenous nature and rare flowering in the Lemnaceae. While characterizing the Spirodela miRNAs and their targets as described in Chapter 1, both Dr. Tang and Dr. Myers noticed a lack of 24nt siRNAs leading to an analysis of the RdDM pathway and transposons. This research coupled with my editing of The Duckweed Genomes textbook led to the writing of Chapters 2, 3, and 4 on small RNAs, transcriptomics, and repetitive DNA elements respectively. While studying the miRNAs and genomics I also developed flowering protocols in order to study the relationship between miR156 and 172 and floral regulation. As described in Chapter 5, I developed Wolffia microscopica, Lemna minor, Lemna gibba, and Spirodela polyrhiza flowering protocols to enable further research. I hope you find this dissertation helpful, and that these flowering protocols, the catalog of miRNAs and their targets, the textbook chapters, and other data contribute to a deeper understanding of this fascinating family, and floral regulation.
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Microbiology and Molecular Genetics
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Supplementary File: Importance of Duckweeds in Basic Research and Their Industrial Applications
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1 online resource (xi, 85 pages)
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Ph.D.
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Includes bibliographical references
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doi:10.7282/t3-s2dx-6v21
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ETD doctoral
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The author owns the copyright to this work.
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Fourounjian
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Paul
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2020-04-28 11:39:50
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Paul Fourounjian
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Rutgers University. School of Graduate Studies
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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