DescriptionApomixis is asexual reproduction via seed. This mode of reproduction can be exploited when an improved genotype is identified, as it can be fixed and reproduced clonally. The problem becomes identifying these novel, improved genotypes. Temporarily disabling apomixis would be an ideal approach to breeding by providing a higher proportion of hybrids to select improved genotypes from. Kentucky bluegrass is a facultative apomict which has led to difficulty in producing improved novel germplasm while retaining a high level of apomictic reproduction, a desired trait in modern cultivars. Through review of literature, it has been hypothesized that ethylene may play a role in the initiation and development of the asexual, apomictic, embryo. To test this hypothesis, ‘Rockstar’ Kentucky bluegrass clones were pollinized by ‘Avalanche’ Kentucky bluegrass following a series of treatments with ethephon in a growth chamber. Four timing levels (Early, pre-culm emergence from the boot; Medium, post-culm emergence from the boot; Late, elongation from the boot; and All timings, all three applications timings in series) and two treatment levels (water control and 1000 ppm ethephon) were tested among three replications for a total of 24 experimental units. A sample of 48 progeny from each experimental unit were subjected to analysis with 10 SSR markers to evaluate the effect of ethephon on reproductive outcomes. No significant difference in the proportion of hybrids was detected as a result of the applications tested.