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Effect of delayed gene therapy treatment in spinal cord injury
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Castro-Pedrido, Sofia. Effect of delayed gene therapy treatment in spinal cord injury. Retrieved from https://doi.org/doi:10.7282/t3-q57s-cv91

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TitleEffect of delayed gene therapy treatment in spinal cord injury
NameCastro-Pedrido, Sofia (author); Cai, Li (chair); Vazquez, Maribel (internal member); Berthiaume, Francois (internal member); Rutgers University; School of Graduate Studies
Date Created2020
Other Date2020-10 (degree)
SubjectDelayed treatment, Biomedical Engineering
Extent1 online resource (vi, 28 pages)
DescriptionGene therapies are being developed which seek to repair the spinal cord and give patients back their mobility by lentivirus-mediated expression of neurogenic transcription factors. Through targeted overexpression of key transcription factors, endogenous cells can be motivated to remodel the site of injury. Previously, our lab has identified a gene therapy in a mouse model of hemisection spinal cord injury (SCI) that, when injected immediately after injury, produces locomotor functional recovery by decreasing cell death, promoting proliferation, and activating neural stem cells. However, in clinical settings, therapeutic intervention would happen hours or even days after the injury. Thus, we aim to determine the effect of a delayed treatment for effective gene therapies of spinal cord injuries. Our hypothesis is that injecting the therapeutic agent some time after injury will still be effective to promote functional recovery. In this study, we used a lateral hemisection SCI mouse model and injected the lentiviral gene therapy one day after injury. The spinal cord tissue was harvested, stained, and analyzed at three different timepoints after the injection to determine the effects of the therapy on the site of injury. Our results showed successful overexpression of the target transcription factor and consequently, decreased cell death in the subacute phase of injury, increased neural stem cell activation, and an increase in cholinergic neurons around the injury site. Our results show that the therapy successfully remodeled the site of injury and could potentially lead to functional recovery in a long-term study. Improving the SCI mouse model for testing gene therapies will better predict their efficacy in clinical settings.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-q57s-cv91
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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