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Role of platelet-derived growth factor-elastin like polypeptide in chronic wound healing
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Chawla, Mehma Kaur. Role of platelet-derived growth factor-elastin like polypeptide in chronic wound healing. Retrieved from https://doi.org/doi:10.7282/t3-49sd-gk36

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TitleRole of platelet-derived growth factor-elastin like polypeptide in chronic wound healing
NameChawla, Mehma Kaur (author); Berthiaume, Francois (chair); Kumar, Suneel (internal member); Schloss, Rene (internal member); Rutgers University; School of Graduate Studies
Date Created2020
Other Date2020-10 (degree)
SubjectBiomedical Engineering
Extent1 online resource (viii, 54 pages)
DescriptionChronic wounds are characterized as non-healing wounds due to poor angiogenesis, impaired vascularization, collagen formation, and dysfunctional fibroblasts and keratinocytes in the hypoxic wound environment. Recent studies have demonstrated the use of growth factors for enhanced wound healing due to their ability to promote proliferation and migration of cells, stimulate collagen synthesis and augment angiogenesis. Platelet-derived growth factor (PDGF) is one of the earliest growth factors to be identified and clinically used for the treatment of chronic wounds. However, their applications are limited because of the increased level of proteases in the hostile wound environment which degrades the growth factors, thus impeding their activity. Here, we have developed and characterized a recombinant fusion protein comprising PDGF and elastin-like polypeptide (ELP). The phase transitioning property of ELP allows rapid purification of the fusion protein using inverse temperature cycling (ITC). The fusion protein retained all characteristics of PDGF-A as evident from fibroblast and endothelial cell proliferation and migration, and endothelial formation of a tube network similar to native PDGF. The self-assembling property of ELP caused the formation of nanoparticles at physiological temperature, which acted as an optimal and stable delivery mechanism for the wound environment. We used 5 nM concentration of PDGF-ELP to show an increase in proliferation by ~2 fold, ~90% wound closure after 48 hours using cell-based scratch assays, and increased angiogenesis demonstrated by enhanced capillary-like tube network formation using a tube assay.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-49sd-gk36
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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