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3D printed spectrophotometer for drug monitoring in tuberculosis patients for global health applications
Descriptive
TitleInfo
Title
3D printed spectrophotometer for drug monitoring in tuberculosis patients for global health applications
Name
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NamePart
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Hooda
NamePart
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Pragya
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Pragya Hooda
Role
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(authority = RULIB)
author
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(type = personal)
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(type = family)
Hassan
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(type = given)
Umer
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Umer Hassan
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Advisory Committee
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(authority = RULIB)
chair
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(type = family)
Pierce
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Mark
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Mark Pierce
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Advisory Committee
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internal member
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(type = family)
Shrirao
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(type = given)
Anil
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Anil Shrirao
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Advisory Committee
Role
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internal member
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Rutgers University
Role
RoleTerm
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degree grantor
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NamePart
School of Graduate Studies
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school
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Text
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theses
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2021
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2021-01
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2021
Language
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(authority = ISO 639-3:2007);
(type = text)
English
Abstract
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Significant progress has been made to fight Tuberculosis (TB), and while many effective diagnoses and treatments have saved millions of lives, the infectious disease still remains to be one of the leading causes of death today. An infected patient can be prescribed with antibiotic drugs such as Rifampin for a certain period of time until treatment is complete. Drug resistant strains of TB have started to grow, creating an urgency to control the spread of this disease. Rapid diagnostic tools and different delivery methods have reduced the mortality rate from TB, however, there is still an urgent need to develop a point-of-care (POC) test that can improve the treatment process for TB patients. Here, we aim to create a POC device that can detect the amount of antibiotic in a biological sample (e.g. urine). We developed and tested a portable three-dimensional (3D) printed spectrophotometer for different biological assays and compared the results with a laboratory standard table-top instrument spectrophotometer. First, we demonstrated the accuracy of the 3D printed spectrophotometer using a Bradford Assay and found a good correlation (R2 = 0.96) between our 3D printed and instrument spectrophotometer. Subsequently, assay is modified to process smaller sample volume using microcuvettes in the setup. We found a correlation coefficient of R2 = 0.92 between our 3D printed and instrument spectrophotometer. The device was then tested for a Rifampin assay and a standard curve is developed in both spiked PBS and urine samples. The correlation results from the rifampin assay will shine light on future diagnostic and drug monitoring testing for TB patients. In conclusion, our results show that our 3D printed spectrophotometer has a strong correlation compared to the real instrument spectrophotometer for absorbance-based measurements. In future, POC instruments such as our 3D printed spectrophotometer can be used by the medical staff for active TB management, and possibly to test patients response to new emerging strains of TB. Proactive diagnosis and treatment of TB patients can significantly reduce the death rate, and in turn improve the quality of human life in global health settings.
Subject
(authority = LCSH)
Topic
Tuberculosis
Subject
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Topic
Biomedical Engineering
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
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(type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier
(type = local)
rucore10001600001
Identifier
ETD_11389
Identifier
(type = doi)
doi:10.7282/t3-5csd-6714
PhysicalDescription
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application/pdf
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text/xml
Extent
1 online resource (vi, 37 pages) : illustrations
Note
(type = degree)
M.S.
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Includes bibliographical references
Location
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(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Hooda
GivenName
Pragya
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime
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2020-12-24 15:39:47
AssociatedEntity
Name
Pragya Hooda
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical
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ETD
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windows xp
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1.5
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2021-01-04T20:46:53
DateCreated
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2021-01-04T20:46:53
ApplicationName
Microsoft® Word 2013
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