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The necessity of tRNA-Val-CAC-1-1 derived tRNA fragments (tRFs) in drosophila
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TitleInfo
Title
The necessity of tRNA-Val-CAC-1-1 derived tRNA fragments (tRFs) in drosophila
Name (type = personal)
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Gattone
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Nicholas
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Nicholas Gattone
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author
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Yakoby
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Nir Yakoby
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Advisory Committee
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Rutgers University
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degree grantor
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Camden Graduate School
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school
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theses
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External ETD graduate
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2021
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2021-01
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English
Abstract
Small non-coding RNAs, such as micro-RNAs (miRNAs) or small interfering RNAs (siRNAs) are critical regulators of gene expression during tissue development. tRNA-derived fragments are a newly discovered class of small non-coding RNA that participate in different biological processes, some of which are attributed to post-transcriptional regulation similar to miRNAs. Using a computational pipeline designed to find tRF targets by mirroring a miRNA-like seed-based targeting mechanism, we identified that the tRNA-Val-CAC-1-1 contains a seed sequence that may target epidermal growth factor receptor (EGFR) inhibitor Sprouty. Using CRISPR/Cas9 genome engineering, this tRNA was deleted. The transgenic fly remained heterozygous, suggesting a necessary role for fly survival. The RNA-seq data from the heterozygous fly did demonstrate small transcriptional changes for target genes in both the ovary and embryo. In addition, a global change in the distribution of small RNAs was found, suggesting a possible stress reaction on the small RNA level from the targeted deletion. Further CRISPR experiments are underway to determine the reason for the unviability of the tRNA-Val-CAC-1-1 homozygous mutant.
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Biology
Subject (authority = LCSH)
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RNA
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Rutgers University Electronic Theses and Dissertations
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Supplementary File: tRF small RNA data
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1 online resource (vii, 36 pages)
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M.S.
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Includes bibliographical references
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Camden Graduate School Electronic Theses and Dissertations
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rucore10005600001
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doi:10.7282/t3-v322-ax10
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Rights

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The author owns the copyright to this work.
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Gattone
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Nicholas
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2021-01-06 12:42:20
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Nicholas Gattone
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Rutgers University. Camden Graduate School
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Copyright protected
Availability
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Open
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Permission or license
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