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Extraction and detection of delta 9-tetrahydrocannabinol from cannabidiol oils using GC/MS
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Fagan, Shavari. Extraction and detection of delta 9-tetrahydrocannabinol from cannabidiol oils using GC/MS. Retrieved from https://doi.org/doi:10.7282/t3-wg1c-tk94

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TitleExtraction and detection of delta 9-tetrahydrocannabinol from cannabidiol oils using GC/MS
NameFagan, Shavari (author); Moran, Kimberlee S (chair); Rutgers University; Camden Graduate School
Date Created2021
Other Date2021-01 (degree)
SubjectExtraction, Forensic Science
Extent1 online resource (v, 36 pages)
DescriptionThe process of detecting delta 9- tetrahydrocannabinol (THC) from CBD oil involves many different factors. Recently, a lot of research has been done in detecting THC in different cannabis products, however very little has been done to detect and even quantify THC from CBD oils. This study set out to validate an already established method and use this method to detect THC from hemp oils that claim not to contain THC on their labels. In order to do this the samples were extracted using a liquid-liquid extraction technique. The method validation consisted of determining the limit of detection (LOD) of CBD and THC and also the precision. Five calibrators were made at different concentrations ranging from 0.1-5 µg/ml. Positive and negative controls were also made to ensure that the experiment was working properly.

Three different blank oils consisting of olive oil, almond oil and coconut oil were used to conduct the validation study. These samples were done in duplicates as well and the instrument used to conduct this study was the gas chromatograph-mass spectrometer (GC-MS). The LOD study was not able to be completed due to time constraints. The lowest concentration of CBD and THC that was analyzed was at 0.1 µg/ml. The retention times of CBD and THC were found to be 12.856 and 13.654 minutes respectively. The percentage coefficient of variation (%CV) was calculated for each oil at their respective concentrations using the peak areas of THC and CBD in order to find precision. It was noted that the majority of the samples had a %CV within 20%, which indicates decent precision; however, some samples were beyond that percentage due to complications in the extraction process creating fluctuations in the peak areas at each concentration analyzed.

After the LOD study was completed 6 different hemp oil samples were extracted and run as well using the same method. Results from these runs found that neither CBD nor THC was not detected in these oil samples. As a result, further studies that need to be done to enhance the extraction procedure and method to make it fully efficient.
NoteM.S.
NoteIncludes bibliographical references
Genretheses, External ETD graduate
Persistent URLhttps://doi.org/doi:10.7282/t3-wg1c-tk94
LanguageEnglish
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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