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The optimization of transformation efficiency in bacterium Chlamydia muridarum

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TitleInfo
Title
The optimization of transformation efficiency in bacterium Chlamydia muridarum
Name (type = personal)
NamePart (type = family)
Hafiz
NamePart (type = given)
Ikram
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Ikram Hafiz
Role
RoleTerm (authority = RULIB)
author
Name (type = personal)
NamePart (type = family)
Runnels
NamePart (type = given)
Loren
DisplayForm
Loren Runnels
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
chair
Name (type = personal)
NamePart (type = family)
Fan
NamePart (type = given)
Huizhou
DisplayForm
Huizhou Fan
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
internal member
Name (type = personal)
NamePart (type = family)
Fondell
NamePart (type = given)
Joseph
DisplayForm
Joseph Fondell
Affiliation
Advisory Committee
Role
RoleTerm (authority = RULIB)
outside member
Name (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB)
degree grantor
Name (type = corporate)
NamePart
School of Graduate Studies
Role
RoleTerm (authority = RULIB)
school
TypeOfResource
Text
Genre (authority = marcgt)
theses
Genre (authority = ExL-Esploro)
ETD graduate
OriginInfo
DateCreated (qualifier = exact); (encoding = w3cdtf); (keyDate = yes)
2021
DateOther (type = degree); (qualifier = exact); (encoding = w3cdtf)
2021-01
Language
LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract (type = abstract)
Chlamydia trachomatis is an obligate intracellular microbe that is responsible for trachoma and chlamydia in much of the developing world, but is difficult to manipulate due to its biphasic developmental cycle. The current protocol for transforming Chlamydia trachomatis is a very time consuming process, yielding limited numbers of successful transformants only after multiple passages of infection which can take up to 48 hours per passage, while also requiring a highly time consuming purification process for elementary bodies. Chlamydia trachomatis’ genetically conserved and faster growing cousin, Chlamydia muridarum (MoPn) was used as a model organism to test the influence of elementary body (EB) purity and concentration of plasmid on transformation efficiency. Ultimately, the experimentation was inconclusive; higher amounts of plasmid in the initial transformation did not yield a higher number of transformants nor yield them in earlier generations when compared to the control groups. Furthermore, the purity of infectious elementary bodies (EBs) were not essential for transformational competence; both ultra-purified EBs that were isolated via a density gradient and partially purified Chlamydia muridarum displayed successful transformants. Further experimentation would be needed with higher sample sizes to ensure statistical significance, and trials on Chlamydia trachomatis itself would have to be performed to ensure consistency across both species. Finally, trimethoprim, banzal-N-acylhydrazones (BAH), and other antibiotics should continue to be screened as alternative selective agents due to their differences in mechanisms of action.
Subject (authority = local)
Topic
Transformation
Subject (authority = local)
Topic
Chlamydia muridarum
Subject (authority = RUETD)
Topic
Physiology and Integrative Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_11471
PhysicalDescription
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application/pdf
InternetMediaType
text/xml
Extent
1 online resource (vii, 22 page) : illustrations
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references
RelatedItem (type = host)
TitleInfo
Title
School of Graduate Studies Electronic Theses and Dissertations
Identifier (type = local)
rucore10001600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/t3-6gzh-0y34
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Rights

RightsDeclaration (ID = rulibRdec0006)
The author owns the copyright to this work.
RightsHolder (type = personal)
Name
FamilyName
Hafiz
GivenName
Ikram
Role
Copyright Holder
RightsEvent
Type
Permission or license
DateTime (encoding = w3cdtf); (qualifier = exact); (point = start)
2021-01-12 12:05:46
AssociatedEntity
Name
Ikram Hafiz
Role
Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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Technical

RULTechMD (ID = TECHNICAL1)
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ETD
OperatingSystem (VERSION = 5.1)
windows xp
DateCreated (point = end); (encoding = w3cdtf); (qualifier = exact)
2021-02-05T14:03:27
CreatingApplication
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1.5
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