LanguageTerm (authority = ISO 639-3:2007); (type = text)
English
Abstract
Despite extensive studies that have been done to understand the regulation of ‘prototypical’ cell morphologies, like the classical rod-shaped E. coli, the regulation of the vast majority of unique, non-symmetric cell shapes remains poorly characterized. Certain bacterial species form thin cellular appendages called stalks or ‘prosthecae’ which are at times used for reproduction, adhesion and other purposes. The Alphaproteobacterium Caulobacter crescentus changes its cell body and stalk length in response to phosphate starvation. The following work uses this model organism to illustrate the molecular underpinnings that enable the organism to adapt to stress by changing its cell length and cellular chemical composition via the remodeling of the cell wall, lipid membranes and overall cellular metabolism.
The stalk cell wall was shown to be distinct due to its ability to evade lysozyme degradation and peptidoglycan protein binding. These observations implied that the stalk’s chemical composition and/or the peptidoglycan geometric configuration leads to the lack of digestion and PG recognition. The stalk PG was found to be crosslinked with LD-transpeptidation as opposed to the more common DD-transpeptidation, this was identified to be mediated predominantly by the LD-transpeptidase LtdD. Consistent with the results seen in Caulobacter, lysozyme resistance was found in other species with peptidoglycan enriched with LD-crosslinks.
During phosphate starvation, a considerable increase in lipid demand occurs as lipid membranes expand due to the stalk elongation. We characterized the increase in the production of non-phosphate derived lipids that are made in order to compensate for that increased demand and found that various anionic glycolipids are largely increased to replace the negatively charged phosphatidylglycerol. In the MS analysis we found a novel lipid species, diglycosylated ceramide (HexHexA-Cer) and identified the entire biosynthesis pathway of this unique lipid. We established that in the absence of ceramide, Caulobacter becomes resistant to the antibiotics polymyxin B and more sensitive to killing by bacteriophage Cr30. Using these two fitness phenotypes we were able to develop a two-step screening method to identify genes responsible for ceramide synthesis. Using our genetic screen, we showed this pathway is widespread throughout bacteria and evolved independently of the eukaryotic ceramide synthesis pathway.
Subject (authority = RUETD)
Topic
Computational and Integrative Biology
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
Identifier
ETD_11360
PhysicalDescription
Form (authority = gmd)
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
Supplementary File: Supplementary Figure 1
Extent
1 online resource (ix, 171 pages)
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Genre (authority = ExL-Esploro)
ETD doctoral
RelatedItem (type = host)
TitleInfo
Title
Camden Graduate School Electronic Theses and Dissertations
Identifier (type = local)
rucore10005600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
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