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Sex differences and consequences of peripheral blood leukocytosis after spinal cord injury in Fischer 344 rats
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Ayala, Carlos. Sex differences and consequences of peripheral blood leukocytosis after spinal cord injury in Fischer 344 rats. Retrieved from https://doi.org/doi:10.7282/t3-mbjq-k583

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TitleSex differences and consequences of peripheral blood leukocytosis after spinal cord injury in Fischer 344 rats
NameAyala, Carlos (author); Young, Wise (chair); Grumet, Martin (internal member); Wu, Long-Jun (internal member); Buckendahl, Patricia (outside member); Rutgers University; School of Graduate Studies
Date Created2021
Other Date2021-01 (degree)
SubjectSpinal cord injury, Neuroscience
Extent1 online resource (xviii, 159 pages)
DescriptionFour times as many men as women have chronic spinal cord injury (SCI), suggesting that women have less severe SCI than men. The mechanism of this large sex gap is not known. SCI changes peripheral blood leukocyte counts, causing acute neutrophilia chronic lymphopenia after injury. Neutrophils infiltrate the injured spinal cord. We compared peripheral blood leukocyte responses in male and female Fischer F344 rats. Males had greater blood neutrophilia than females during the first days after SCI. We also measured myeloperoxidase (MPO) by Western Blot injured spinal cords. Surprisingly, MPO did not differ between male and female rats, despite greater peripheral neutrophilia in males. We assessed effects of blood leukocyte responses on locomotor recovery. We treated male and female rats with the chemotherapeutic agent cyclophosphamide (CYP) 2 days before SCI to reduce acute blood neutrophilia and evaluated locomotor recovery and neuronal and myelin sparing in the spinal cords at 6 weeks after injury. CYP prevented increases in blood neutrophil counts after SCI. However, blood neutrophil counts did not correlate with tissue damage or behavioral recovery. Male rats recovered walking earlier than female rats regardless of blood neutrophilia. Differences in recovery were transient and not significant 3 to 6 weeks after
SCI. Quantitative analyses of the rat spinal cords showed that standardized contusion injury caused similar tissue loss, myelin sparing, and neuron survival in both sexes. However, male spinal cords were bigger in age-matched 100-day-old F344 rats. Preventing acute blood neutrophil responses did not change walking recovery or tissue damage in either sex. These findings argue strongly against the hypothesis that acute neutrophilia is responsible for sex differences in recovery after SCI. We conclude that acute blood leukocytosis do not affect recovery or tissue damage after spinal cord injury in F344 rats.
NotePh.D.
NoteIncludes bibliographical references
Genretheses, ETD doctoral
Persistent URLhttps://doi.org/doi:10.7282/t3-mbjq-k583
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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