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Sex differences and consequences of peripheral blood leukocytosis after spinal cord injury in Fischer 344 rats
Descriptive
TitleInfo
Title
Sex differences and consequences of peripheral blood leukocytosis after spinal cord injury in Fischer 344 rats
Name
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Ayala
NamePart
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Carlos
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Carlos Ayala
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author
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(type = personal)
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(type = family)
Young
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Wise
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Wise Young
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Advisory Committee
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chair
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(type = personal)
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Grumet
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Martin
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Martin Grumet
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Advisory Committee
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(authority = RULIB)
internal member
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(type = personal)
NamePart
(type = family)
Wu
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(type = given)
Long-Jun
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Long-Jun Wu
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Advisory Committee
Role
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(authority = RULIB)
internal member
Name
(type = personal)
NamePart
(type = family)
Buckendahl
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(type = given)
Patricia
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Patricia Buckendahl
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Advisory Committee
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(authority = RULIB)
outside member
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Rutgers University
Role
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(authority = RULIB)
degree grantor
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School of Graduate Studies
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theses
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2021
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2021-01
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English
Abstract
Four times as many men as women have chronic spinal cord injury (SCI), suggesting that women have less severe SCI than men. The mechanism of this large sex gap is not known. SCI changes peripheral blood leukocyte counts, causing acute neutrophilia chronic lymphopenia after injury. Neutrophils infiltrate the injured spinal cord. We compared peripheral blood leukocyte responses in male and female Fischer F344 rats. Males had greater blood neutrophilia than females during the first days after SCI. We also measured myeloperoxidase (MPO) by Western Blot injured spinal cords. Surprisingly, MPO did not differ between male and female rats, despite greater peripheral neutrophilia in males. We assessed effects of blood leukocyte responses on locomotor recovery. We treated male and female rats with the chemotherapeutic agent cyclophosphamide (CYP) 2 days before SCI to reduce acute blood neutrophilia and evaluated locomotor recovery and neuronal and myelin sparing in the spinal cords at 6 weeks after injury. CYP prevented increases in blood neutrophil counts after SCI. However, blood neutrophil counts did not correlate with tissue damage or behavioral recovery. Male rats recovered walking earlier than female rats regardless of blood neutrophilia. Differences in recovery were transient and not significant 3 to 6 weeks after
SCI. Quantitative analyses of the rat spinal cords showed that standardized contusion injury caused similar tissue loss, myelin sparing, and neuron survival in both sexes. However, male spinal cords were bigger in age-matched 100-day-old F344 rats. Preventing acute blood neutrophil responses did not change walking recovery or tissue damage in either sex. These findings argue strongly against the hypothesis that acute neutrophilia is responsible for sex differences in recovery after SCI. We conclude that acute blood leukocytosis do not affect recovery or tissue damage after spinal cord injury in F344 rats.
SCI. Quantitative analyses of the rat spinal cords showed that standardized contusion injury caused similar tissue loss, myelin sparing, and neuron survival in both sexes. However, male spinal cords were bigger in age-matched 100-day-old F344 rats. Preventing acute blood neutrophil responses did not change walking recovery or tissue damage in either sex. These findings argue strongly against the hypothesis that acute neutrophilia is responsible for sex differences in recovery after SCI. We conclude that acute blood leukocytosis do not affect recovery or tissue damage after spinal cord injury in F344 rats.
Subject
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Topic
Spinal cord injury
Subject
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Topic
Neuroscience
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Rutgers University Electronic Theses and Dissertations
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ETD_11457
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application/pdf
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text/xml
Extent
1 online resource (xviii, 159 pages)
Note
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Ph.D.
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Includes bibliographical references
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ETD doctoral
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School of Graduate Studies Electronic Theses and Dissertations
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rucore10001600001
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NjNbRU
Identifier
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doi:10.7282/t3-mbjq-k583
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Rights
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The author owns the copyright to this work.
RightsHolder
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Name
FamilyName
Ayala
GivenName
Carlos
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Permission or license
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2021-01-06 16:26:44
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Name
Carlos Ayala
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Copyright holder
Affiliation
Rutgers University. School of Graduate Studies
AssociatedObject
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
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2021-01-12T00:21:08
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2021-01-12T18:16:11
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1.4
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