Description4-hydroxystyrene is a phenolic chemical compound used in polymer and flavor industries. Microbial biosynthesis of this compound has been studied over recent years as a sustainable green alternative to synthetic chemical production. In this current study, 4-hydroxystyrene was biosynthesized using engineered E. coli. To improve the hydroxystyrene biosynthesis utilizing the carbon source following strategies were adopted. The pathway for bioproduction was bifurcated, and the co-culture of E. coli was implemented over monoculture. An amino acid exporter protein PhpCAT was utilized in combination biosensor-assisted cell selection mechanism to maximize the pathway intermediate tyrosine's biosynthesis. The exporter improved tyrosine's mass transfer between the co-culture members, and the resulting co-culture produced 298 mg/L 4-hydroxystyrene from 5 g/L glucose, which is 96% higher than the control co-culture without the exporter. The achieved yield of 0.06 g/g glucose is the highest among previous studies on de novo microbial biosynthesis. It shows that the exporter protein's utilization and biosensors enhance the pathway intermediate in a co-culture study and boost the hydroxystyrene biosynthesis performance. This strategy can pave the path for microbial biosynthesis for all the potential aromatic-derived compounds.