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Icelandic bdelloidea transcriptomic profiling through differential expression analysis
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Snow, Ryan. Icelandic bdelloidea transcriptomic profiling through differential expression analysis. Retrieved from https://doi.org/doi:10.7282/t3-g8sw-j471

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TitleIcelandic bdelloidea transcriptomic profiling through differential expression analysis
NameSnow, Ryan (author); Shain, Daniel (chair); Oberle, Jennifer (member); Klein, Eric (member); Geneva, Anthony (member); Rutgers University; Graduate School - Camden
Date Created2021
Other Date2021-10 (degree)
SubjectBiology, Bioinformatics, Adaptation, Bdelloidea, Cold, RNA-Seq, Rotifer, Transcriptome
Extent1 online resource (x, 65 pages)
DescriptionWith the recent upsurge of Bdelloidea discoveries in permanently cold environments, members of the group represent prime candidates for better understanding adaptations to one of the most pervasive abiotic stressors; cold. Previous studies have highlighted the role of inducing cold stress tolerance through manipulation of the purine and energy metabolic pathways. Genomic alterations to these regions have been evaluated to offset the molecular and cellular dysfunctions associated with cold exposure (i.e. decreased molecular motion) by increasing intracellular adenylate pool levels. Here, through means of differential expression analysis, the recently discovered Icelandic glacial bdelloids (family Philodinidae) are compared against the current bdelloid reference; Adineta vaga. The intent is to identify any members of the purine and energy metabolic pathways exhibit changes in expression that may induce cold tolerance. The assessment of differentially expressed genes shared across all Icelandic bdelloid samples revealed two genes of interest suggested to play a role in cold tolerance; ADK1 and AT5G. Predicted to encode for adenylate kinase and ATP synthase lipid-binding protein [mitochondrial], respectively. As well, an overabundance of genes encoding ribosomal proteins and dynein heavy chain were discovered within the top differentially expressed genes with relative expression values shared across all Icelandic samples. Furthermore, gene enrichment analysis of the differentially expressed datasets provided insight into the prime components, processes, and functions impacted by changes in expression. Giving way to one of, if not the first, transcriptomic profiling of members from family Philodinidae.
NoteM.S.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-g8sw-j471
LanguageEnglish
CollectionCamden Graduate School Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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