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Desulfoluna spongiiphila strain AA1 and other marine sponge associated reductive debrominating bacteria
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Lopez, Nora A.. Desulfoluna spongiiphila strain AA1 and other marine sponge associated reductive debrominating bacteria. Retrieved from https://doi.org/doi:10.7282/t3-6bvp-c461

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TitleDesulfoluna spongiiphila strain AA1 and other marine sponge associated reductive debrominating bacteria
NameLopez, Nora A. (author); Kerkhof, Lee (chair); Bromberg, Yana (member); Fennell, Donna (member); Rutgers University; School of Graduate Studies
Date Created2021
Other Date2021-10 (degree)
SubjectMicrobiology, Genetics, Molecular biology, Bacteria, Brominated organics, Marine sponges, Reductive debromination, Reductive dehalogenase
Extent1 online resource (xii, 121 pages) : illustrations
DescriptionAnaerobic microorganisms associated with marine sponges can reductively dehalogenate organohalide compounds. Desulfoluna spongiiphila strain AA1 isolated from the marine sponge Aplysina aerophoba is an anaerobic bacterium capable of growth by respiratory dehalogenation. To investigate the presence of D. spongiiphila within other marine sponges and the potential ability for in situ dehalogenation, specimens of Pseudaxinella lunaecharta, Chondrilla nucula and Clathria prolifera were examined. Sponges amended with 2,6-dibromophenol (at 100 µM) demonstrated dehalogenation activity at a rate of over 1.5 mM day-1 kg sponge-1 for P. lunaecharta and C. prolifera. Desulfoluna spongiiphila-like bacteria were also found within these sponge tissues. To determine the genes associated with debromination, the genome of Desulfoluna spongiiphila strain AA1 was sequenced, and three putative reductive dehalogenase genes were identified. Initially, the reductive dehalogenase enzyme was found to be a corrinoid dependent enzyme through propyl iodide reversible inhibition. Then, a search of corrinoid binding motifs in open reading frames yielded a number of putative reductive dehalogenase genes each containing the two conserved motifs found in most reductive dehalogenases: e.g., a tat signal sequence and iron-sulfur clusters. Each of these putative dehalogenase genes was associated with a downstream gene encoding for the potential membrane anchoring protein and a gene for a transcriptional regulator. A transcription assay with D. spongiiphila strain AA1 was attempted to verify involvement of the putative genes in debromination. In summary, marine sponges have the potential for dehalogenation in the environment and D. spongiiphila-like bacteria may be involved in this process in different sponge species. The putative gene(s) encoding the reductive dehalogenase genes of Desulfoluna spongiiphila strain AA1 have been uncovered.
NotePh.D.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-6bvp-c461
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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