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Anaerobic molybdenum-cofactor–utilizing bacteria: genomic insights into anaerobic respiration and anaerobic (halo)benzoate degradation
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Louie, Tiffany S.. Anaerobic molybdenum-cofactor–utilizing bacteria: genomic insights into anaerobic respiration and anaerobic (halo)benzoate degradation. Retrieved from https://doi.org/doi:10.7282/t3-2b1k-yr47

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TitleAnaerobic molybdenum-cofactor–utilizing bacteria: genomic insights into anaerobic respiration and anaerobic (halo)benzoate degradation
NameLouie, Tiffany S. (author); Häggblom, Max (chair); Lee, Patrick KH (member); Kerkhof, Lee J (member); Young, Lily Y (member); Fennell, Donna E (member); Rutgers University; School of Graduate Studies
Date Created2021
Other Date2021-10 (degree)
SubjectMicrobiology, Anaerobic bacteria -- Genetics, Microbial metabolism, Molybdenum enzymes, Biodegradation, Anaerobic respiration, Molybdoenzymes
Extent1 online resource (xiii, 112 pages) : illustrations
DescriptionBacteria can harvest energy from a range of carbon sources from simple to complex, and couple this to the anaerobic respiration of different terminal electron acceptors. Bacterial metabolism has a major impact on the biogeochemical cycling of the elements involved. The objective of this dissertation, was to investigate the genetic code behind the diverse metabolic capabilities of three different anaerobic bacteria — Sedimenticola selenatireducens AK4OH1, Thauera chlorobenzoica 3CB-1, and Seleniivibrio woodruffii S4 — specifically those encoding the enzymes involved in the degradation of aromatic compounds and molybdenum-containing enzymes involved in oxidation-reduction reactions.

The anaerobic degradation of aromatic compounds often involves coenzyme A (CoA) ligases to form the electron-withdrawing CoA-thioester group on the aromatic ring to prepare the aromatic system for reduction by CoA reductases. The genomic analyses revealed the CoA ligase and CoA reductase genes encoded within S. selenatireducens AK4OH1 and T. chlorobenzoica 3CB-1, both of which are capable of anaerobic mineralization of different benzoic acids derivatives.

Anaerobic respiration with terminal electron acceptors such as nitrate, selenate, and arsenate is catalyzed by molybdenum-containing enzymes (molybdoenzymes) that are part of the dimethylsulfoxide reductase (DMSOR) family. S. selenatireducens AK4OH1, T. chlorobenzoica 3CB-1, and S. woodruffii S4 all utilize DMSORs for their anaerobic respiration, and this was evidenced in their genomes. Only a limited number of DMSORs were found in T. chlorobenzoica 3CB-1, which was only previously determined to respire nitrate. In contrast, S. selenatireducens AK4OH1, which could respire nitrate, nitrite, and selenate had many more DMSORs than expected, but still paled in comparison to the record number found in S. woodruffii S4.
NotePh.D.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-2b1k-yr47
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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