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Pyrrole-imidazole polyamide-H promotes anti-inflammation during the acute phase of spinal cord injury
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Esteban, Fatima C.. Pyrrole-imidazole polyamide-H promotes anti-inflammation during the acute phase of spinal cord injury. Retrieved from https://doi.org/doi:10.7282/t3-rxm3-af85

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TitlePyrrole-imidazole polyamide-H promotes anti-inflammation during the acute phase of spinal cord injury
NameEsteban, Fatima C. (author); Cai, Li (chair); Schloss, Rene (member); Berthiaume, Francois (member); Rutgers University; School of Graduate Studies
Date Created2022
Other Date2022-05 (degree)
SubjectEngineering, Inflammation, Minocycline, Pyrrole-imidazole polyamide, Spinal cord injury
Extent39 pages : illustrations
DescriptionSpinal cord injuries (SCI) cause severe primary damage to the central nervous system during impact. Secondary damage continues to develop due to an imbalanced microenvironment. Interleukin-6 (IL-6) is responsible for recruiting other proinflammatory cytokines and acute phase proteins to the injury site. However, an excess of proinflammatory signaling can result in increased inflammation, axonal retraction, fibrosis, and extended wound healing. Pyrrole-imidazole polyamides (PIPs) are DNA sequence-selective small molecules capable of gene regulation. One of such molecules, PIP-H, displayed a 4 times greater downregulation for IL-6. The purpose of this study is to test the efficacy of PIP-H at reducing the pro-inflammatory cytokine and its subsequent effects on the microenvironment during the acute phase of injury. Adult C57BL/6 mice were subject to a moderate contusion SCI and treatment (saline, minocycline-hydrochloride, or PIP-H) immediately at the lesion site after injury. At 1 day post-injury, the animals were sacrificed for ELISA or immunohistochemistry analysis. Results show that PIP-H treatment significantly decreases the expression of IL-6, CD68, and GFAP as compared to saline and minocycline treatments. This study supports the use of PIP-H as an effective inhibitor of IL-6 gene expression. Downregulating the proinflammatory cytokine is believed to positively influence the microenvironment during the acute phase of injury.
NoteM.S.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-rxm3-af85
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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