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Investigating sintered porous polycaprolactone scaffolds in reducing bone production
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Paul, Nikhil Ashok. Investigating sintered porous polycaprolactone scaffolds in reducing bone production. Retrieved from https://doi.org/doi:10.7282/t3-2ate-ev66

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TitleInvestigating sintered porous polycaprolactone scaffolds in reducing bone production
NamePaul, Nikhil Ashok (author); Labazzo, Kristen (chair); Freeman, Joseph (member); Dunn, Michael (member); Rutgers University; School of Graduate Studies
Date Created2022
Other Date2022-05 (degree)
SubjectBiomedical engineering, Tissue scaffolds
Extent70 pages : illustrations
DescriptionHeterotopic Ossification (HO) is a unique pathological condition involving the formation of extraneous bone in the muscle and other soft tissues often seen in amputees. This is particularly problematic in the military where soldiers may lose their limbs from a blast injury. There are currently no ideal treatments for HO, and usually a second surgery is needed. Often, the amputee won’t be able to wear their prosthesis anymore, reducing quality of life. To address these uncomfortable ectopic growths, we have aspired to develop a novel approach, in which we take traditional tissue engineering practices that are normally designed for encouraging bone growth, and instead use this knowledge to achieve the opposite objective by fabricating a scaffold that will appropriately heal, but then discourage, any problematic bone growth. This work describes the fabrication of electro-spun polymeric scaffolds that have decreasing pore size with increasing depth. Because gradients in pore size have been shown to have a discernible effect upon bone cell differentiation, and smaller pore sizes naturally limit the movement of cells and their necessary nutrients, the scaffold fabrication process used varying porogen sizes to create the desired gradient (2). This was accomplished by introducing salt into the electro-spun scaffolds. The salt crystals ranged in size from 100-700 μm, and are introduced individually into single layers. After the electrospinning process, the polymer layers were sintered together so that the pore size decreases from one end to the other. SEM was used to verify the structure, and the scaffolds were examined for their growth-limiting ability in vitro using pre-osteoblast cells in conjunction with assays designed to ascertain the presence of any differentiation markers, and overall cell viability. Ultimately, the data revealed that each scaffold group provided a 3-dimensional framework capable of maintaining cell viability, and are potential candidates for reducing bone precursor cell differentiation.
NoteM.S.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-2ate-ev66
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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