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Portable 3D printed multispectral sensing of enzyme activity in real-time based on absorbance spectroscopy
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Jesuraj, Antony Michael Jenitter. Portable 3D printed multispectral sensing of enzyme activity in real-time based on absorbance spectroscopy. Retrieved from https://doi.org/doi:10.7282/t3-k6eh-8z58

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TitlePortable 3D printed multispectral sensing of enzyme activity in real-time based on absorbance spectroscopy
NameJesuraj, Antony Michael Jenitter (author); Hassan, Umer (chair); Javanmard, Mehdi (member); Wu, Chung-Tse Michael (member); Rutgers University; School of Graduate Studies
Date Created2023
Other Date2023-01 (degree)
SubjectElectrical engineering, Enzymes -- Spectroscopic imaging, Spectrophotometry
Extent63 pages : illustrations
DescriptionTyrosinase is a critical enzyme responsible for melanin synthesis in living beings and, exhibiting enzymatic browning in fruits and vegetables. It finds various commercial applications in fields of healthcare (skin pigmentation, wound healing), forensic, and food processing. It is also responsible for biochemical sclerotization process. Tyrosinase activity is dependent on various factors such as enzyme concentration, substrate concentration used, temperature, operating pH, and other regulators such as inhibitors and effectors. Hence it is critical to monitor the enzyme activity under different conditions while using the enzyme. The activity of tyrosinase is measured in different ways e.g., by using Atomic Force Microscope (AFM), where the AFM tip is coated with the substrate used in the catalytic process and the force measured corresponds to the activity. Other measurement techniques include electrochemical, colorimetry, spectrophotometry, and spectroscopy. Most of these techniques typically require laboratory equipment that are bulky, high power, complex to operate and fail to be used in portable and space constraint application environments, and most importantly for real-time on-site monitoring. To address these challenges, here, we developed a portable 3D printed multispectral spectrophotometer for real-time monitoring of enzyme molecular activity. The device measures absorbance spectrum at multiple spectrum channels for a fixed excitation source in transmission mode. The synchronized device consists of optical filters with Gaussian characteristics with a defined bandwidth at the detector. The detector, and excitation source are controlled by a microcontroller that is responsible for data acquisition and processing. For tyrosinase activity monitoring, the 3D printed device has shown significant correlation (R2 = 0.999) with the measurements obtained from the standard table-top spectrophotometer for enzyme activity ranging in between 0.18 ΔAmin−1 to 0.016 ΔAmin−1. The enzyme activity was also studied using the 3D printed spectrophotometer while using kojic acid, a common commercially employed tyrosinase inhibitor while varying both its molar concentration and volume concentration to control the reaction rate at discrete activity levels. The device measures an average correlation of 0.9983 for the inhibition study measurements resulting in the lowest reduction of the enzyme activity to about 0.002 ΔAmin−1, for each of the reaction rates against the standard table-top spectrophotometer. This miniaturized 3D printed spectrophotometer is about 95% reduced dimension and weight compared to the reference table-top spectrophotometer used in this study.
NoteM.S.
NoteIncludes bibliographical references
Genretheses
Persistent URLhttps://doi.org/doi:10.7282/t3-k6eh-8z58
LanguageEnglish
CollectionSchool of Graduate Studies Electronic Theses and Dissertations
Organization NameRutgers, The State University of New Jersey
RightsThe author owns the copyright to this work.
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