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Stabilization of irradiated allografts via crosslinking and free radical scavenging
Descriptive
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Title
Stabilization of irradiated allografts via crosslinking and free radical scavenging
Name
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Seto
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Aaron U.
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Aaron U. Seto
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author
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Dunn
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Michael
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Advisory Committee
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Michael Dunn
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chair
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Gatt
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Charles
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Advisory Committee
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Charles Gatt
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internal member
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Parsons
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JR
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Advisory Committee
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JR Parsons
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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school
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theses
OriginInfo
DateCreated
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2007
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2007
Language
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English
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electronic
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application/pdf
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text/xml
Extent
xi, 99 pages
Abstract
Currently, the risk of disease transmission from allografts has been reduced through a system of screening methods organized by tissue banks. Terminal sterilization is possible with the use of ionizing irradiation, which has shown high efficiency for neutralizing pathogens. Unfortunately, irradiation is known to cause chain scission and crosslink degradation in collagen, leading to weakened mechanical properties and increased susceptibility to enzymatic digestion.
Two methods of stabilizing allografts against the effects of irradiation were studied in this research. These methods included crosslinking via EDC or glucose, and free radical scavenging using mannitol, ascorbate, or riboflavin. The overall objective of this study was to assess the protective ability of each treatment in the presence of increasing irradiation dose based on mechanical properties and enzyme digestion. These studies also investigated the influence of tendon water content, and combination of crosslink and scavenging methods after exposure to irradiation. Our hypothesis was that crosslinking and free radical scavenging would aid in maintaining mechanical integrity and enzymatic resistance after gamma or ebeam irradiation.
In general, crosslinking and free radical scavenging improved the mechanical properties and collagenase resistance of irradiated tendons. Glucose crosslinked tendons irradiated at 25 kGy were comparable in terms of strength to native tendon. Similarly, at 50 kGy EDC crosslinked tendons were comparable to untreated tendons irradiated at 25 kGy. Ascorbate and riboflavin were successful at protecting mechanical properties especially at 25 kGy. Most noteworthy were the combined treatments irradiated at 50 kGy, which matched native tendons mechanically and were highly resistive to collagenase.
These treatments were unable to completely maintain properties at 50 kGy, which is closer to a clinically useful dose. The majority of treatments displayed improvements at 25 kGy. If allografts could be successfully stabilized from damage, ionizing irradiation could ensure not only disease free tissues, but also faster availability.
Two methods of stabilizing allografts against the effects of irradiation were studied in this research. These methods included crosslinking via EDC or glucose, and free radical scavenging using mannitol, ascorbate, or riboflavin. The overall objective of this study was to assess the protective ability of each treatment in the presence of increasing irradiation dose based on mechanical properties and enzyme digestion. These studies also investigated the influence of tendon water content, and combination of crosslink and scavenging methods after exposure to irradiation. Our hypothesis was that crosslinking and free radical scavenging would aid in maintaining mechanical integrity and enzymatic resistance after gamma or ebeam irradiation.
In general, crosslinking and free radical scavenging improved the mechanical properties and collagenase resistance of irradiated tendons. Glucose crosslinked tendons irradiated at 25 kGy were comparable in terms of strength to native tendon. Similarly, at 50 kGy EDC crosslinked tendons were comparable to untreated tendons irradiated at 25 kGy. Ascorbate and riboflavin were successful at protecting mechanical properties especially at 25 kGy. Most noteworthy were the combined treatments irradiated at 50 kGy, which matched native tendons mechanically and were highly resistive to collagenase.
These treatments were unable to completely maintain properties at 50 kGy, which is closer to a clinically useful dose. The majority of treatments displayed improvements at 25 kGy. If allografts could be successfully stabilized from damage, ionizing irradiation could ensure not only disease free tissues, but also faster availability.
Note
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M.S.
Note
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Includes bibliographical references (p. 80-85).
Subject
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Topic
Biomedical Engineering
Subject
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Topic
Irradiation
Subject
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Topic
Homografts
Subject
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Topic
Transplantation of organs, tissues, etc.
Subject
(ID = SUBJ5);
(authority = ETD-LCSH)
Topic
Free radicals (Chemistry)
Subject
(ID = SUBJ6);
(authority = ETD-LCSH)
Topic
Crosslinking (Polymerization)
RelatedItem
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16776
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ETD_443
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Identifier
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doi:10.7282/T38C9WPH
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ETD graduate
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The author owns the copyright to this work.
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Copyright protected
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Open
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Aaron Seto
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Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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