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The wound response in Arabidopsis thaliana and Physcomitrella patens
Descriptive
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Title
The wound response in Arabidopsis thaliana and Physcomitrella patens
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Tang
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Chi-Tai Conan
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Chi-Tai Conan Tang
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author
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Lawton
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Michael
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Advisory Committee
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Michael A Lawton
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chair
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Belanger
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Advisory Committee
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Faith Belanger
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Gianfagna
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Tom
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Advisory Committee
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Tom Gianfagna
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internal member
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Raskin
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Ilya
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Advisory Committee
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Ilya Raskin
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internal member
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Cohick
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Wendie
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Advisory Committee
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Wendie Cohick
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outside member
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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theses
OriginInfo
DateCreated
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2007
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2007
Language
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English
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electronic
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application/pdf
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text/xml
Extent
xviii, 229 pages
Abstract
The wound response in plants is triggered by tissue damage incurred from biotic and abiotic sources. However, identification of wound-specific genes has been complicated by overlap between the wound response and other stress responses. Using representational difference analysis (RDA), an anthocyanidin synthase (ANS), steroid sulfotransferase (SST) and a tyramine transferase (THT) were isolated from wounded Arabidopsis thaliana. These genes were expressed in wounded leaves and corresponding systemic tissues. Analysis in wild-type and mutant backgrounds showed that their wound induction was entirely dependent upon jasmonate signaling. SST and putative paralog At5g07000 possess sequence hallmarks of steroid sulfotransferases. The expression of SST was silenced using RNAi. A silenced (SST::RNAi) line was analyzed for differences in steroid profiles between unwounded and wounded plants. However, differences were not detected between wild-type and SST::RNAi plants. Possible reasons for this result are discussed. An RDA-based screen for wounding genes expressed solely in systemic tissues (SYSTs) revealed, instead, genes whose expression is down-regulated in injured leaves. SYST1-3 was depleted in wounded leaves within five minutes after wounding and maintained at reduced levels for at least four days. Genetic approaches failed to identify the signal involved in SYST1-3 reduction. A SYST1-3-like gene, At5g16250, was isolated by homology search and was similarly depleted in wounded leaves indicating a gene family of wound-depleted transcripts. To gain insight into the wound response of a lower eukaryotic plant, the wound response was investigated in the moss, Physcomitrella patens. Wounding caused cell death to 1-2 cell layers bordering the wound. Callose was deposited in cells adjacent to the wounded cells. Two putative lipoxygenases (PpLOX) were induced by wounding. PpLOX6 and PpLOX7 contain features of lipoxygenases from higher plants. PpLOX7 is predicted to be transported into the chloroplast and a dendrogram showed that PpLOX7 is related to the wound-induced type2-lipoxygenases whereas PpLOX6 clustered with the moss lipoxygenases. Induction of PpLOX7 could be triggered by methyl jasmonate. Wounding also induced putative orthologs of JA biosynthetic enzymes, allene oxide synthase (AOS) and allene oxide cyclase (AOC) but not an OPDA reductase (OPR3). These results suggest that a JA-dependent wound response may be conserved in mosses.
Note
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Ph.D.
Note
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Includes bibliographical references (p. 191-229).
Subject
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Topic
Plant Biology
Subject
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Topic
Plants--Wounds and injuries
Subject
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Topic
Arabidopsis thaliana
Subject
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Topic
Physcomitrella patens
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.16786
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ETD_312
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NjNbRU
Identifier
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doi:10.7282/T32V2GHM
Genre
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ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
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Name
Chi-Tai Tang
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Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Non-exclusive ETD license
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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