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Expression and analysis of ricin A chain in Saccharomyces cerevisiae
Descriptive
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Title
Expression and analysis of ricin A chain in Saccharomyces cerevisiae
Name
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Baricevic
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Marianne Michelle
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Marianne Michelle Baricevic
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author
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Cohick
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Wendie
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Advisory Committee
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Wendie Cohick
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chair
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Tumer
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Nilgun
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Advisory Committee
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Nilgun Tumer
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internal member
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White
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Lori
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Advisory Committee
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Lori White
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internal member
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Di
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Rong
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Advisory Committee
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Rong Di
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internal member
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Gunderson
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Samuel
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Advisory Committee
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Samuel Gunderson
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outside member
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Rutgers University
Role
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degree grantor
Name
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NamePart
Graduate School - New Brunswick
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school
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Text
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theses
OriginInfo
DateCreated
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2008
DateOther
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2008-05
Language
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English
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electronic
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application/pdf
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text/xml
Extent
vii, 94 pages
Abstract
Ricin is a ribosome inactivating protein (RIP) isolated from ricinus communis, the castor bean plant. RIPs catalytically depurinate an adenine residue from the highly conserved sarcin/ricin loop in the large ribosomal RNA subunit, rendering the ribosome unable to translate protein. Due to its potential use as a bioweapon, understanding how ricin gains access to and depurinates ribosomes is of high importance. There is currently no approved vaccine or treatment for ricin intoxication. Learning the residues that are critical for ricin toxicity and enzymatic activity may help to generate a potential vaccine for ricin exposure. Here, I describe an analysis of ricin A chain (RTA), the enzymatic subunit of ricin, in Saccharomyces cerevisiae. The results provide evidence that ricin cytotoxicity is not necessarily a result of ribosome depurination and translation inhibition, ricin utilizes components of the ER Association Degradation (ERAD) pathway to reach the cytosol from the ER and the C-terminus of RTA is essential for enzymatic activity and protein translocation across the ER membrane.
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references (p. 87-92).
Subject
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Topic
Microbiology and Molecular Genetics
Subject
(ID = SUBJ2);
(authority = ETD-LCSH)
Topic
Ricin
Subject
(ID = SUBJ3);
(authority = ETD-LCSH)
Topic
Ribosomes
Subject
(ID = SUBJ4);
(authority = ETD-LCSH)
Topic
Saccharomyces cerevisiae--Genetics
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.17275
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ETD_922
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T390245J
Genre
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ETD doctoral
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Rights
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
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Name
Marianne Baricevic
Role
Copyright holder
Affiliation
Rutgers University. Graduate School - New Brunswick
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Permission or license
Detail
Non-exclusive ETD license
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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