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Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Using molecular techniques to quantify iron bound nitrogenase in trichodesmium IMS 101 and natural populations
SubTitle
PartName
PartNumber
NonSort
Identifier
ETD_1107
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000050470
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Oceanography
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Trichodesmium
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Nitrogenase
Abstract
Iron has been widely recognized as a potentially key factor in promoting nitrogen fixation by Trichodesmium. Data from both laboratory and field studies demonstrates that increasing the iron concentrations stimulates growth, photosynthetic rates and nitrogen fixation of both cultured and natural populations. However, quantitative studies that elucidate relationships between cellular iron quotas and physiological mechanisms have been limited. In this study, molecular techniques enabled quantification of the amount of nitrogenase expressed in iron-replete cultures of Trichodesmium IMS 101 over a diel cycle. A standard of the purified iron component of nitrogenase was generated from an expression and protein purification system. Using this standard and known values of intracellular carbon, the amount of nitrogenase per carbon at peak expression was measured, 0.038 mg nitrogenase: mg C. The quantity of iron bound in the nitrogenase structure was then calculated; Fe:C equal to 236.53 [mu]mol: mol. Using estimates of Trichodesmium biomass from the literature, the amount of iron bound in the nitrogenase structure was calculated for various ocean regions, resulting in 2.22 [mu]mol m-3 and 0.05 [mu]mol m-3 of iron bound in nitrogenase in the subtropical North Atlantic and North Pacific, respectively.
PhysicalDescription
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electronic resource
Extent
viii, 32 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 29-32)
Note (type = statement of responsibility)
by Sherrie Whittaker
Name (ID = NAME-1); (type = personal)
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Whittaker
NamePart (type = given)
Sherrie
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author
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Sherrie Whittaker
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Falkowski
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Paul
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chair
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Advisory Committee
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Paul Falkowski
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Schofield
NamePart (type = given)
Oscar
Role
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internal member
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Advisory Committee
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Oscar M.E. Schofield
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Bidle
NamePart (type = given)
Kay
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Kay Bidle
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2008
DateOther (qualifier = exact); (type = degree)
2008-10
Place
PlaceTerm (type = code)
xx
Location
PhysicalLocation (authority = marcorg)
NjNbRU
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = doi)
doi:10.7282/T31J9B1X
Genre (authority = ExL-Esploro)
ETD graduate
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The author owns the copyright to this work.
Copyright
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Copyright protected
Availability
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Open
RightsEvent (AUTHORITY = rulib); (ID = 1)
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Permission or license
Detail
Non-exclusive ETD license
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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ETD
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application/pdf
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application/x-tar
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1239040
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