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An integrated biomarker approach for assessing exposure and effects of endocrine disruptors and other contaminants in killifish (Fundulus heteroclitus) from the New York-New Jersey harbor estuary

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
An integrated biomarker approach for assessing exposure and effects of endocrine disruptors and other contaminants in killifish (Fundulus heteroclitus) from the New York-New Jersey harbor estuary
SubTitle
PartName
PartNumber
NonSort
Identifier
ETD_1736
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051182
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Environmental Sciences
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Killifishes
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Mummichog
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Fishes--Effect of water pollution on
Abstract
A multi-tiered approach was used to evaluate killifish (Fundulus heteroclitus) health by examining a suite of biomarkers in killifish inhabiting the heavily industrialized Newark Bay and a reference population in Tuckerton, NJ. The general hypothesis of this study was that Newark Bay killifish would exhibit biomarker responses indicative of impaired health when compared to a reference population from Tuckerton, NJ. The biomarkers investigated included classical endpoints (histopathology, morphometrics, gonad maturation), hepatic mRNA expression (CYP1A, metallothionein, vitellogenin I), gonadal aromatase mRNA expression, hepatic protein levels (CYP1A and vitellogenin I) and chemical exposure analyses (bile PAHs). Newark Bay fish had significantly higher levels of bile PAHs compared to reference fish. Females had significantly higher concentrations of naphthalene, pyrene and benzo[a]pyrene (3, 6 and 4 fold higher, respectively, p<0.05). Males had significantly higher concentrations of pyrene (7-fold higher, p<0.05), and higher concentrations of naphthalene (4-fold higher, p=0.06) and benzo[a]pyrene (9-fold higher, p=0.07). Histological lesions of the liver and pancreas in Newark Bay fish were similar to reference fish. Newark Bay fish had significantly higher expression of hepatic CYP1A for both males and females (7 and 3 fold higher, respectively, p<0.05) and CYP1A protein levels for both males and females (5 and 8 fold higher, p<0.05). Endocrine disruption in male gonads is demonstrated by a decreased gonad weight, altered testis development and upregulated aromatase expression (3-fold, p<0.05), which indicates exposure to endocrine active compounds. Efforts to strip spawn Newark Bay females produced few viable eggs, while at the same time Tuckerton females produced large numbers of viable eggs. There was a significant increase in the percent of pre-vitellogenic follicles (43% at Tuckerton, 64% at Newark Bay) and a significantly decreased percent of follicles at the mid-vitellogenic and mature stages (25% at Tuckerton, 3% at Newark Bay) in Newark Bay females (p<0.05). Vitellogenin mRNA and protein (egg yolk-protein) was significantly decreased in Newark Bay females (6-fold lower mRNA, 27-fold lower protein, p<0.05) while gonadal aromatase (produces 17β-estradiol) was significantly increased (210-fold higher, p<0.05). Killifish in the NY-NJ Harbor Estuary are exposed to high amounts of PAHs and aryl hydrocarbon agonists and their reproductive health is impaired. Impaired reproductive health is possibly due to disruption of steroid signaling by aryl hydrocarbon receptor-estrogen receptor crosstalk leading to decreased vitellogenin production.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
xiii, 100 p. : ill.
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application/pdf
InternetMediaType
text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 91-100)
Note (type = statement of responsibility)
by Sean M. Bugel
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Bugel
NamePart (type = given)
Sean M.
NamePart (type = date)
1981
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author
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Sean M. Bugel
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Cooper
NamePart (type = given)
Keith
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chair
Affiliation
Advisory Committee
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Keith R. Cooper
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Burger
NamePart (type = given)
Joanna
Role
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internal member
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Advisory Committee
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Joanna Burger
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Reinfelder
NamePart (type = given)
John
Role
RoleTerm (authority = RULIB); (type = )
internal member
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Advisory Committee
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John Reinfelder
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
White
NamePart (type = given)
Lori
Role
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outside member
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Advisory Committee
DisplayForm
Lori A. White
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-05
Place
PlaceTerm (type = code)
xx
Location
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NjNbRU
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Identifier (type = doi)
doi:10.7282/T3348KK3
Genre (authority = ExL-Esploro)
ETD graduate
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Open
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Non-exclusive ETD license
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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