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Development of anti-inflammatory agents from the aromatic plants, Origanum spp. and Mentha spp., and analytical methods on the quality control of bioactive phenolic compounds
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Development of anti-inflammatory agents from the aromatic plants, Origanum spp. and Mentha spp., and analytical methods on the quality control of bioactive phenolic compounds
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ETD_1182
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051767
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eng
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theses
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Medicinal Chemistry
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Topic
Anti-inflammatory agents
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Topic
Oregano--Therapeutic use
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(authority = ETD-LCSH)
Topic
Mints (Plants)--Therapeutic use
Abstract
The development of new anti-inflammatory drugs comes at high cost and risk, as evidenced by the fact that several novel prescription non-steroidal anti-inflammatory drugs (NSAIDs) have been withdrawn from the market due to potential health risks. Meanwhile, natural dietary supplements with anti-inflammatory activities are gaining increased interest by consumers and industry as they are perceived to be of lower cost, with fewer adverse effects and yet function as complementary and alternative medicines. We successfully identified the anti-inflammatory constituents from post-distillation
material of oregano, an aromatic plant long prized as a food seasoning and spice in western and Middle Eastern cuisine and a rich source of essential oil. The anti-inflammatory compounds in oregano were identified as rosmarinic acid, oleanolic acid and ursolic acid by using bioactivity-guided isolation, LC/MS and NMR techniques. We subsequently elucidated their possible inhibitory mechanisms during the inflammatory cell signaling pathways by screening different bioassays, and a synergistic action for the combination of these three compounds was observed on the LPS-induced nitrite production assay. An LC/MS (SIM mode) method employing a tandem column system was developed for the simultaneous quantitation of these three compounds in oregano. Validation for this method showed a precision (relative standard deviation) ranging from 4.84% to 6.41%, and the recoveries varied from 92.2% to 100.8% for the three analytes. In order to investigate genetic diversity between species and within a species relative to the anti-inflammatory compounds, different oregano species, varieties and breeding lines were collected from around the world, vegetatively field transplanted into a Rutgers Agricultural Research Experiment Station farm, where the plants were field grown, harvested and quantitated for their anti-inflammatory contents based upon the LC/MS method developed in this study. We next examed mint, another aromatic plant in the Lamiaceae family, and proved that these three anti-inflammatory compounds also accumulated in this plant. An analytical survey was further performed to determine anti-inflammatory agent-rich sources from eight commercial mint species as well as many specialty varieties within the different species. To facilitate the frequent quality control requirement and material pre-evaluation, we developed and validated a near-infrared spectroscopy (NIR) method for the fast quantitation of anti-inflammatory constituents in oregano and mint. Finally, we investigated the anti-inflammatory compound distribution and related chemical properties of the oregano post-distillation materials.
material of oregano, an aromatic plant long prized as a food seasoning and spice in western and Middle Eastern cuisine and a rich source of essential oil. The anti-inflammatory compounds in oregano were identified as rosmarinic acid, oleanolic acid and ursolic acid by using bioactivity-guided isolation, LC/MS and NMR techniques. We subsequently elucidated their possible inhibitory mechanisms during the inflammatory cell signaling pathways by screening different bioassays, and a synergistic action for the combination of these three compounds was observed on the LPS-induced nitrite production assay. An LC/MS (SIM mode) method employing a tandem column system was developed for the simultaneous quantitation of these three compounds in oregano. Validation for this method showed a precision (relative standard deviation) ranging from 4.84% to 6.41%, and the recoveries varied from 92.2% to 100.8% for the three analytes. In order to investigate genetic diversity between species and within a species relative to the anti-inflammatory compounds, different oregano species, varieties and breeding lines were collected from around the world, vegetatively field transplanted into a Rutgers Agricultural Research Experiment Station farm, where the plants were field grown, harvested and quantitated for their anti-inflammatory contents based upon the LC/MS method developed in this study. We next examed mint, another aromatic plant in the Lamiaceae family, and proved that these three anti-inflammatory compounds also accumulated in this plant. An analytical survey was further performed to determine anti-inflammatory agent-rich sources from eight commercial mint species as well as many specialty varieties within the different species. To facilitate the frequent quality control requirement and material pre-evaluation, we developed and validated a near-infrared spectroscopy (NIR) method for the fast quantitation of anti-inflammatory constituents in oregano and mint. Finally, we investigated the anti-inflammatory compound distribution and related chemical properties of the oregano post-distillation materials.
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electronic resource
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xvii, 281 p. : ill.
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Ph.D.
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Includes bibliographical references
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by Diandian Shen
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Diandian Shen
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Simon
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James
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James E. Simon
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LaVoie
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Edmond
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Edmond J. LaVoie
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Ho
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Chi-Tang
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Chi-Tang Ho
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Wu
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Qing-Li
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Qing-Li Wu
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Hartman
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Thomas
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Thomas G. Hartman
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Rutgers University
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Graduate School - New Brunswick
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2008
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2008-10
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xx
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Rutgers University Electronic Theses and Dissertations
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Graduate School - New Brunswick Electronic Theses and Dissertations
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doi:10.7282/T31N81BW
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ETD doctoral
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The author owns the copyright to this work.
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Copyright protected
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Open
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Shen
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Diandian
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Diandian Shen
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Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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