RUcore: Rutgers University Community Repository
Development of a fiber-reinforced meniscus scaffold
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Development of a fiber-reinforced meniscus scaffold
SubTitle
PartName
PartNumber
NonSort
Identifier
(displayLabel = );
(invalid = )
ETD_2188
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051781
Language
(objectPart = )
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Biomedical Engineering
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Meniscus (Anatomy)--Wounds and injuries--Treatment
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Knee--Wounds and injuries--Treatment
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Tissue engineering
Abstract
The meniscus provides protection to the articular cartilage of the knee by transmitting loads through the joint, distributing high peak stresses on the underlying surfaces, providing shock absorption, and aiding in joint lubrication. It is well accepted that significant loss of meniscal tissue leads to degenerative changes in the joint. Treatment alternatives for patients suffering from severe meniscal deficiency are limited, and thus far have not been shown to offer long-term protection to the underlying cartilage.
This dissertation describes the development of a tissue engineered meniscus scaffold comprised of a chemically crosslinked type I collagen sponge reinforced with resorbable polymer fibers. The long-term goal of this work is to develop a resorbable scaffold which promotes the growth of fibrocartilaginous-like tissue while preventing or delaying degenerative changes in the underlying articular surfaces.
Preliminary evaluation was completed on several potential designs varying in fiber orientation, collagen sponge density, and overall geometry. Two were chosen to evaluate a series of hypotheses related to their biomechanical properties, in vitro biocompatibility, and in vivo biocompatibility. Both designs were found to possess the structural properties necessary to function as a load-bearing device in the knee. Furthermore, they were fabricated from resorbable materials which supported the proliferation of fibrochondrocytes in vitro. A non-functional evaluation in a rabbit model demonstrated the scaffold to elicit a biological response appropriate for a resorbable device. From the results of these experiments, one design was chosen for functional evaluation in a large animal model.
Scaffolds were implanted at the site of a total meniscectomy in a sheep knee. Short-term results demonstrated that scaffolds incorporated into the joint and elicited an appropriate biological response. However, observed neo-tissue did not possess the high organization inherent to fibrocartilaginous tissues. Furthermore, scaffolds were found to have a limited protective effect on the articular cartilage as variable levels of degenerative changes were observed for all subjects. Results from this evaluation showed proof of principle for this type of scaffold for the treatment of significant meniscal deficiency. However, further optimization of the device is required before it can proceed to clinical evaluation.
This dissertation describes the development of a tissue engineered meniscus scaffold comprised of a chemically crosslinked type I collagen sponge reinforced with resorbable polymer fibers. The long-term goal of this work is to develop a resorbable scaffold which promotes the growth of fibrocartilaginous-like tissue while preventing or delaying degenerative changes in the underlying articular surfaces.
Preliminary evaluation was completed on several potential designs varying in fiber orientation, collagen sponge density, and overall geometry. Two were chosen to evaluate a series of hypotheses related to their biomechanical properties, in vitro biocompatibility, and in vivo biocompatibility. Both designs were found to possess the structural properties necessary to function as a load-bearing device in the knee. Furthermore, they were fabricated from resorbable materials which supported the proliferation of fibrochondrocytes in vitro. A non-functional evaluation in a rabbit model demonstrated the scaffold to elicit a biological response appropriate for a resorbable device. From the results of these experiments, one design was chosen for functional evaluation in a large animal model.
Scaffolds were implanted at the site of a total meniscectomy in a sheep knee. Short-term results demonstrated that scaffolds incorporated into the joint and elicited an appropriate biological response. However, observed neo-tissue did not possess the high organization inherent to fibrocartilaginous tissues. Furthermore, scaffolds were found to have a limited protective effect on the articular cartilage as variable levels of degenerative changes were observed for all subjects. Results from this evaluation showed proof of principle for this type of scaffold for the treatment of significant meniscal deficiency. However, further optimization of the device is required before it can proceed to clinical evaluation.
PhysicalDescription
Form
(authority = gmd)
electronic resource
Extent
xiv, 172 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references (p. 157-168)
Note
(type = statement of responsibility)
by Eric Andrew Balint
Name
(ID = NAME-1);
(type = personal)
NamePart
(type = family)
Balint
NamePart
(type = given)
Eric Andrew
NamePart
(type = date)
1979
Role
RoleTerm
(authority = RULIB);
(type = )
author
DisplayForm
Eric Andrew Balint
Name
(ID = NAME-2);
(type = personal)
NamePart
(type = family)
Gatt
NamePart
(type = given)
Charles
Role
RoleTerm
(authority = RULIB);
(type = )
chair
Affiliation
Advisory Committee
DisplayForm
Charles J Gatt
Name
(ID = NAME-3);
(type = personal)
NamePart
(type = family)
Dunn
NamePart
(type = given)
Michael
Role
RoleTerm
(authority = RULIB);
(type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Michael G Dunn
Name
(ID = NAME-4);
(type = personal)
NamePart
(type = family)
Langrana
NamePart
(type = given)
Noshir
Role
RoleTerm
(authority = RULIB);
(type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Noshir Langrana
Name
(ID = NAME-5);
(type = personal)
NamePart
(type = family)
Jaffe
NamePart
(type = given)
Michael
Role
RoleTerm
(authority = RULIB);
(type = )
outside member
Affiliation
Advisory Committee
DisplayForm
Michael Jaffe
Name
(ID = NAME-1);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB);
(type = )
degree grantor
Name
(ID = NAME-2);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB);
(type = )
school
OriginInfo
DateCreated
(point = );
(qualifier = exact)
2009
DateOther
(qualifier = exact);
(type = degree)
2009-10
Place
PlaceTerm
(type = code)
xx
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
PhysicalLocation
(authority = marcorg);
(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3D79BKD
Genre
(authority = ExL-Esploro)
ETD doctoral
Back to the top
Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Balint
GivenName
Eric
Role
Copyright holder
RightsEvent
(ID = RE-1);
(AUTHORITY = rulib)
Type
Permission or license
Label
Place
DateTime
Detail
AssociatedEntity
(ID = AE-1);
(AUTHORITY = rulib)
Role
Copyright holder
Name
Eric Balint
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
(ID = AO-1);
(AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top
Technical
ContentModel
ETD
MimeType
(TYPE = file)
application/pdf
MimeType
(TYPE = container)
application/x-tar
FileSize
(UNIT = bytes)
13015040
Checksum
(METHOD = SHA1)
07f783005e9175dd4fbbe59282e7419fdeed3462
Back to the top
Statistical Profile