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Requirement for a core 1 galactosyltransferase in the Drosophila nervous system
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Title
Requirement for a core 1 galactosyltransferase in the Drosophila nervous system
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ETD_2118
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051861
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eng
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theses
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Topic
Cell and Developmental Biology
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(authority = ETD-LCSH)
Topic
Galactosyltransferases
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Drosophila
Abstract
Glycosylation is important in a lot of fundamental biological processes, including cell recognition, cell adhesion, and cell signaling. Mucin-type O-glycosylation involves the synthesis of glycoproteins, expressed in mucous secretions and as transmembrane proteins on the cell surfaces. However, the biological functions of mucin-type O-glycans remain incompletely understood. I have pursued genetic and biochemical studies to understand their importance during development in Drosophila.
Mucin-type O-glycosylation is initiated by the attachment of N-acetylgalactosamine (GalNAc) to Ser or Thr residues, and then elongated by additional sugars. To examine the requirements for mucin-type glycosylation in Drosophila, I characterized the expression and phenotypes of core 1 galactosyltransferases (core 1 GalTs), which elongate O-GalNAc by adding galactose in a β1, 3 linkage. Among Drosophila core 1 GalTs, CG9520 (C1GalTA) is expressed in the amnioserosa and central nervous system. A null mutation in C1GalTA is lethal. The mutant animals show a morphogenetic defect in their central nervous system in which the ventral nerve cord is greatly elongated and the brain hemispheres are distorted. Lectin staining and blotting experiments confirmed that C1GalTA is required for the synthesis of Gal-ß1,3-GalNAc in vivo. Our observations establish a role for mucin-type O-glycosylation during neural development in Drosophila.
Overexpression of C1GalTA causes a wing blistering phenotype, which occurs when adhesion between the two ventral and dorsal surfaces of the wing blade is lost, and is also commonly seen in integrin mutants. This result implicates mucin-type O-glycans in cell adhesion in the Drosophila wing blade.
Altogether, these results suggest a role of mucin-type O-glycosylation in Drosophila development, including the morphogenesis of central nervous system and the formation of the wing blade.
Mucin-type O-glycosylation is initiated by the attachment of N-acetylgalactosamine (GalNAc) to Ser or Thr residues, and then elongated by additional sugars. To examine the requirements for mucin-type glycosylation in Drosophila, I characterized the expression and phenotypes of core 1 galactosyltransferases (core 1 GalTs), which elongate O-GalNAc by adding galactose in a β1, 3 linkage. Among Drosophila core 1 GalTs, CG9520 (C1GalTA) is expressed in the amnioserosa and central nervous system. A null mutation in C1GalTA is lethal. The mutant animals show a morphogenetic defect in their central nervous system in which the ventral nerve cord is greatly elongated and the brain hemispheres are distorted. Lectin staining and blotting experiments confirmed that C1GalTA is required for the synthesis of Gal-ß1,3-GalNAc in vivo. Our observations establish a role for mucin-type O-glycosylation during neural development in Drosophila.
Overexpression of C1GalTA causes a wing blistering phenotype, which occurs when adhesion between the two ventral and dorsal surfaces of the wing blade is lost, and is also commonly seen in integrin mutants. This result implicates mucin-type O-glycans in cell adhesion in the Drosophila wing blade.
Altogether, these results suggest a role of mucin-type O-glycosylation in Drosophila development, including the morphogenesis of central nervous system and the formation of the wing blade.
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electronic resource
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viii, 95 p. : ill.
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Ph.D.
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Includes bibliographical references (p. 90-94)
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by Yuh-Ru Lin
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Lin
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Yuh-Ru
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Yuh-Ru Lin
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Ruth
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Ruth Steward
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Irvine
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Kenneth
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Kenneth Irvine
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Rongo
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Christopher
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Christopher Rongo
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Kramer
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Sunita
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Sunita Kramer
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Rutgers University
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Graduate School - New Brunswick
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2009
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2009-10
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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doi:10.7282/T3930T9W
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ETD doctoral
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The author owns the copyright to this work
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Copyright protected
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Open
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Lin
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Yuh-Ru
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Yuh-Ru Lin
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Rutgers University. Graduate School - New Brunswick
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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