Staff View
Requirement for a core 1 galactosyltransferase in the Drosophila nervous system

Descriptive

TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Requirement for a core 1 galactosyltransferase in the Drosophila nervous system
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2118
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051861
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Cell and Developmental Biology
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Galactosyltransferases
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Drosophila
Abstract
Glycosylation is important in a lot of fundamental biological processes, including cell recognition, cell adhesion, and cell signaling. Mucin-type O-glycosylation involves the synthesis of glycoproteins, expressed in mucous secretions and as transmembrane proteins on the cell surfaces. However, the biological functions of mucin-type O-glycans remain incompletely understood. I have pursued genetic and biochemical studies to understand their importance during development in Drosophila.
Mucin-type O-glycosylation is initiated by the attachment of N-acetylgalactosamine (GalNAc) to Ser or Thr residues, and then elongated by additional sugars. To examine the requirements for mucin-type glycosylation in Drosophila, I characterized the expression and phenotypes of core 1 galactosyltransferases (core 1 GalTs), which elongate O-GalNAc by adding galactose in a β1, 3 linkage. Among Drosophila core 1 GalTs, CG9520 (C1GalTA) is expressed in the amnioserosa and central nervous system. A null mutation in C1GalTA is lethal. The mutant animals show a morphogenetic defect in their central nervous system in which the ventral nerve cord is greatly elongated and the brain hemispheres are distorted. Lectin staining and blotting experiments confirmed that C1GalTA is required for the synthesis of Gal-ß1,3-GalNAc in vivo. Our observations establish a role for mucin-type O-glycosylation during neural development in Drosophila.
Overexpression of C1GalTA causes a wing blistering phenotype, which occurs when adhesion between the two ventral and dorsal surfaces of the wing blade is lost, and is also commonly seen in integrin mutants. This result implicates mucin-type O-glycans in cell adhesion in the Drosophila wing blade.
Altogether, these results suggest a role of mucin-type O-glycosylation in Drosophila development, including the morphogenesis of central nervous system and the formation of the wing blade.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
viii, 95 p. : ill.
InternetMediaType
application/pdf
InternetMediaType
text/xml
Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references (p. 90-94)
Note (type = statement of responsibility)
by Yuh-Ru Lin
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Lin
NamePart (type = given)
Yuh-Ru
Role
RoleTerm (authority = RULIB); (type = )
author
DisplayForm
Yuh-Ru Lin
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Steward
NamePart (type = given)
Ruth
Role
RoleTerm (authority = RULIB); (type = )
chair
Affiliation
Advisory Committee
DisplayForm
Ruth Steward
Name (ID = NAME-3); (type = personal)
NamePart (type = family)
Irvine
NamePart (type = given)
Kenneth
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Kenneth Irvine
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Rongo
NamePart (type = given)
Christopher
Role
RoleTerm (authority = RULIB); (type = )
internal member
Affiliation
Advisory Committee
DisplayForm
Christopher Rongo
Name (ID = NAME-5); (type = personal)
NamePart (type = family)
Kramer
NamePart (type = given)
Sunita
Role
RoleTerm (authority = RULIB); (type = )
outside member
Affiliation
Advisory Committee
DisplayForm
Sunita Kramer
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-10
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
PhysicalLocation (authority = marcorg); (displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier (type = doi)
doi:10.7282/T3930T9W
Genre (authority = ExL-Esploro)
ETD doctoral
Back to the top

Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Lin
GivenName
Yuh-Ru
Role
Copyright holder
RightsEvent (ID = RE-1); (AUTHORITY = rulib)
Type
Permission or license
Label
Place
DateTime
Detail
AssociatedEntity (ID = AE-1); (AUTHORITY = rulib)
Role
Copyright holder
Name
Yuh-Ru Lin
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (ID = AO-1); (AUTHORITY = rulib)
Type
License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
Back to the top

Technical

ContentModel
ETD
MimeType (TYPE = file)
application/pdf
MimeType (TYPE = container)
application/x-tar
FileSize (UNIT = bytes)
20992000
Checksum (METHOD = SHA1)
78e979fa0705b44563667f3343dd0e8c36919fe9
Back to the top
Version 8.3.13
Rutgers University Libraries - Copyright ©2021