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Nuclear organization of mouse Hox cluster paralogs during mouse embryonic stem cell differentiation to neural stem cell
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Title
Nuclear organization of mouse Hox cluster paralogs during mouse embryonic stem cell differentiation to neural stem cell
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ETD_2053
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051888
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eng
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theses
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Topic
Biomedical Engineering
Subject
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(authority = ETD-LCSH)
Topic
Homeobox genes
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Embryonic stem cells--Differentiation
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Neural stem cells
Abstract
In this project we use Hox genes as a genetic tool to understand how nuclear architecture regulates cell differentiation during embryonic development. Hox genes come under the category of homeobox genes, a highly evolutionarily conserved group of genes with an important role during embryogenesis. Hox genes are located on 4 distinct chromosomes, in cluster paralogs (HOX A, B, C, D). Each individual cluster contains up to 13 homologous genes and corresponding genes on different clusters (e.g., HoxA13, HoxD13) exhibit varying degrees of functional redundancy. The position of a gene in the cluster is related to its spatiotemporal pattern of expression along the anterior-posterior axis of the embryo. The coordination of the spatiotemporal expression of equivalent paralog group genes on different clusters/chromosomes is coordinated is still not known. Our primary hypothesis is that nuclear architecture defines a regulatory framework of Hox cluster loci in the nucleus when the Hox cluster transcription is activated and maintained. We did a comparative analysis on the Hox cluster nuclear architecture in mouse embryonic stem cells (ESCs) and fibroblast growth factors (FGF) - induced differentiation to neural stem cells (NSC). We show for the first time that Hox gene expression is induced by FGF treatment in vitro simultaneously in the four Hox cluster. Using three-dimensional confocal fluorescence microscopy, FISH and computational techniques, we mapped the position of Hox gene cluster paralogs in individual nuclei of both cell types. We did not observe nuclear colocalization of Hox heterologous cluster in NSC. However, we observe that heterologous clusters tend to occupy similar nuclear domains in NSC, which may favor undetected long-range gene interactions. Nevertheless, our results indicate that Hox gene cluster nuclear three-dimensional organization is neither random nor correlated to the changes in nuclear volume and shape that parallel cell differentiation.
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electronic resource
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viii, 55 p. : ill.
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M.S.
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Includes bibliographical references (p. 53-55)
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by Priya Panicker
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Panicker
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Priya
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1973-
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Priya Panicker
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Tischfield
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Dr.Jay
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chair
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Advisory Committee
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Dr.Jay Tischfield
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Shreiber
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Dr. David
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Advisory Committee
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Dr. David Shreiber
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Serrano
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Dr.Lourdes
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outside member
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Advisory Committee
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Dr.Lourdes Serrano
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Rutgers University
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degree grantor
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Graduate School - New Brunswick
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2009
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2009-10
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xx
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Rutgers University Electronic Theses and Dissertations
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ETD
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Graduate School - New Brunswick Electronic Theses and Dissertations
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rucore19991600001
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doi:10.7282/T3X0676B
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ETD graduate
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Rights
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The author owns the copyright to this work
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Copyright protected
Notice
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Open
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Permission or license
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Panicker
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Priya
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Priya Panicker
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Rutgers University. Graduate School - New Brunswick
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Author Agreement License
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I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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819200
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