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The role of dissolved organic matter in structuring microbial community composition

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
The role of dissolved organic matter in structuring microbial community composition
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_2016
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000051907
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Oceanography
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Aquatic ecology
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Biotic communities
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Microbial growth
Subject (ID = SBJ-5); (authority = ETD-LCSH)
Topic
Water--Organic compound content
Abstract
Dissolved organic matter (DOM) is an important source of nutrients in aquatic systems contributing to the growth of phytoplankton and bacteria. The overall response appears to be driven by the phytoplankton and bacteria species present as well as the composition of DOM provided. This dissertation explores the bioavailability of allochthonous and autochthonous DOM sources to, and its affect on, the growth of marine phytoplankton and bacterial community abundance and composition.
This research utilizes advanced chemical (electrospray ionization mass spectrometry (ESI-MS)) and molecular (terminal restriction fragment length polymorphism (TRFLP)) techniques to characterize the DOM and microbial community. To investigate the role of allochthonous DOM in phytoplankton growth, DOM from two different riverine sources from watersheds with different land use practices, was supplied to a natural cyanobacteria population. The bioavailability of autochthonous DOM was investigated by supplying DOM produced by a single culture of cyanobacteria to a natural dinoflagellate bloom community. The potential negative effect of an individual autochthonous DOM compound was investigated through the addition of marine toxin, brevetoxin, to three different natural bacterial communities.
This dissertation resulted in the first ESI-MS characterization spectra of the DOM associated with three different natural phytoplankton blooms, a culture of cyanobacteria, and two different South Florida rivers. It was also the first study to identify previously uncharacterized allochthonous and autochthonous DOM masses bioavailable to natural marine phytoplankton communities. Bulk level analyses within these experiments quantified lower limits for the bioavailability of allochthonous and autochthonous DOM sources and the relative community response to each of these sources. This dissertation also represents the first molecular evaluation of the bacteria associated with a bloom and the first investigation of the allelopathic properties of brevetoxin. It has discussed and applied the use of ESI-MS to investigate the bioavailability of complex DOM, identified and quantified potential nutrient sources and linked marine toxin production to changes in bacterial community composition.
PhysicalDescription
Form (authority = gmd)
electronic resource
Extent
xii, 179 p. : ill.
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application/pdf
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Note (type = degree)
Ph.D.
Note (type = bibliography)
Includes bibliographical references
Note (type = statement of responsibility)
by Rachel Elizabeth Sipler
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Sipler
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Rachel Elizabeth
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1981-
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author
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Rachel Elizabeth Sipler
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Seitzinger
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Sybil
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chair
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Advisory Committee
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Sybil P Seitzinger
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Schofield
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Oscar
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co-chair
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Advisory Committee
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Oscar M Schofield
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Bidle
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Kay
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internal member
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Advisory Committee
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Kay D Bidle
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Kirkpatrick
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Gary
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outside member
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Advisory Committee
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Gary J Kirkpatrick
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Glibert
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Patricia
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outside member
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Advisory Committee
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Patricia M Glibert
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
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degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-10
Place
PlaceTerm (type = code)
xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
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TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3RF5V5W
Genre (authority = ExL-Esploro)
ETD doctoral
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Rights

RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Sipler
GivenName
Rachel
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Copyright holder
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Name
Rachel Sipler
Affiliation
Rutgers University. Graduate School - New Brunswick
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical

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ETD
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application/pdf
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application/x-tar
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