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Influence of immunoenhancement by dietary vitamin E supplementation on the development of Listeria monocytogenes infection in aged and young guinea pigs

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TypeOfResource
Text
TitleInfo (ID = T-1)
Title
Influence of immunoenhancement by dietary vitamin E supplementation on the development of Listeria monocytogenes infection in aged and young guinea pigs
SubTitle
PartName
PartNumber
NonSort
Identifier (displayLabel = ); (invalid = )
ETD_1486
Identifier (type = hdl)
http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000052176
Language (objectPart = )
LanguageTerm (authority = ISO639-2); (type = code)
eng
Genre (authority = marcgt)
theses
Subject (ID = SBJ-1); (authority = RUETD)
Topic
Food Science
Subject (ID = SBJ-2); (authority = ETD-LCSH)
Topic
Vitamin E--Therapeutic use
Subject (ID = SBJ-3); (authority = ETD-LCSH)
Topic
Immune response
Subject (ID = SBJ-4); (authority = ETD-LCSH)
Topic
Listeria monocytogenes
Abstract
L. monocytogenes is a facultative intracellular pathogen of humans and animals. Although L. monocytogenes is responsible for approximately 2500 cases of foodborne illnesses in the United States, it results in 88- 92 % of hospitalizations attributed to foodborne pathogens and causes an associated high mortality rate of 25- 40 %. Due to the natural decline of the immune system, infectious disease is one of the major causes of mortality in the elderly. Orthomolecular levels of vitamin E have been shown to promote T cell proliferation in healthy aged animals and human. We investigated the impact of immunomodulation on the development of listerial infection within an aged and young population after low dose challenge with L. monocytogenes. Animals were immunoenhanced with daily supplementation of vitamin E for a period of 21 days. An untreated control group was included. Blood samples were immunostained, and changes in the level of CD8+ and CD3+ T cells were determined using flow cytometry analysis. Animals were orally challenged with 100 CFU of L. monocytogenes. Daily supplementation with vitamin E increased the level of total (CD3+) T cells and cytotoxic (CD8+) T cells significantly in young but not old animals. Results showed that 8 % and 15 % of young and aged control animals respectively, became infected; whereas, 0 % and 13 % of young and aged animals, respectively, receiving orthomolecular doses of vitamin E became infected. During post-challenge period, vitamin E treated aged animals showed a faster CD8+ T cell proliferation response than control aged animals. Results also showed that dietary vitamin E supplementation boosted a pool of T cells. In conclusion, experiments demonstrate the effect of vitamin E in mitigating listerial infection in young animals; and stimulating immune response in young and aged animals.
PhysicalDescription
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electronic resource
Extent
xii, 64 p. : ill.
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application/pdf
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text/xml
Note (type = degree)
M.S.
Note (type = bibliography)
Includes bibliographical references (p. 58-64)
Note (type = statement of responsibility)
by Wen-Hsuan Wu
Name (ID = NAME-1); (type = personal)
NamePart (type = family)
Wu
NamePart (type = given)
Wen-Hsuan
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author
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Wen-Hsuan Wu
Name (ID = NAME-2); (type = personal)
NamePart (type = family)
Matthews
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Karl
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chair
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Advisory Committee
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Karl R. Matthews
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NamePart (type = family)
Schaffner
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Donald
Role
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co-chair
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Advisory Committee
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Donald W. Schaffner
Name (ID = NAME-4); (type = personal)
NamePart (type = family)
Chikindas
NamePart (type = given)
Mikhael
Role
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co-chair
Affiliation
Advisory Committee
DisplayForm
Mikhael L. Chikindas
Name (ID = NAME-1); (type = corporate)
NamePart
Rutgers University
Role
RoleTerm (authority = RULIB); (type = )
degree grantor
Name (ID = NAME-2); (type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm (authority = RULIB); (type = )
school
OriginInfo
DateCreated (point = ); (qualifier = exact)
2009
DateOther (qualifier = exact); (type = degree)
2009-01
Place
PlaceTerm (type = code)
xx
RelatedItem (type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier (type = RULIB)
ETD
RelatedItem (type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier (type = local)
rucore19991600001
Location
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NjNbRU
Identifier (type = doi)
doi:10.7282/T3PR7W43
Genre (authority = ExL-Esploro)
ETD graduate
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RightsDeclaration (AUTHORITY = GS); (ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Notice
Note
Availability
Status
Open
Reason
Permission or license
Note
RightsHolder (ID = PRH-1); (type = personal)
Name
FamilyName
Wu
GivenName
Wen-Hsuan
Role
Copyright Holder
RightsEvent (AUTHORITY = rulib); (ID = RE-1)
Type
Permission or license
Label
Place
DateTime
2009-01-05 02:02:38
Detail
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Role
Copyright holder
Name
Wen-Hsuan Wu
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject (AUTHORITY = rulib); (ID = AO-1)
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License
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Place
DateTime
Detail
365 days
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ETD
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application/pdf
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application/x-tar
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1259520
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