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Identification of candidate anti-obesity targets
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Identification of candidate anti-obesity targets
Identifier
ETD_2490
Identifier
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http://hdl.rutgers.edu/1782.2/rucore10001600001.ETD.000053154
Language
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(type = code)
eng
Genre
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theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Cell and Developmental Biology
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Obesity
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Reducing diets
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Weight loss
Subject
(ID = SBJ-5);
(authority = ETD-LCSH)
Topic
Obesity--Treatment
Abstract
(type = abstract)
Over the past decade, obesity has become a major health issue in the western world, and is very rapidly becoming a concern for eastern countries as well. In the most basic sense, obesity results from an imbalance between energy intake and energy expenditure. However, the complex disease that is obesity arises as a result of the insufficient energy consumption, resulting in adiposity, the increase of triglyceride storage in adipocytes, body weight gain, and an increased risk of obesity co-morbidities. As the prevalence of obesity is expected to increase over the next 20 years, the identification of novel anti-obesity targets has become paramount in the search for an efficacious and compliance-friendly obesity therapy. In an effort to identify and classify potential anti-obesity targets as experimental candidates for obesity therapy, WAT (white adipose tissue) from Hmga2-/-, Lepob.Lepob double knockout mice, Hmga2+/+ mice, Hmga2-/- mice, and Lepob/Lepob mice were run on microarray to determine gene expression profiles for each genotype. Exclusion criteria were added, and a list of 138 adipocyte-specific genes remained. The genes were then characterized through bioinformatics according to 6 parameters: 1) chromosomal location in mouse genome; 2) chromosomal location in human genome; 3) subcellular localization; 4) function; 5) knockout phenotype in mice; 6) obesity-related QTLs in human genome. After characterization, each gene was evaluated in greater detail and filtered to form a list of candidates for experimental analysis to evaluate anti-obesity effectiveness. The list of 138 genes was finally narrowed to 10 genes, 6 of which were present in the top 25 most highly expressed genes in adipocyte-rich WAT. Preliminary experimental analysis reveals diminished weight gain and overall decreased body weight in mice upon peptide inhibition for one gene from the filtered list. While we have only begun evaluating one gene, thus far, the results are encouraging in that the method used to develop and filter such a list of genes is proving to be validated. Further experimental research is required in order to fully validate each potential candidate; however such an approach, to determine solid candidates, appears promising.
PhysicalDescription
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electronic resource
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ix, 69 p. : ill.
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Note
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M.S.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = statement of responsibility)
by Ahmed Hussain Rizvi
Note
Includes abstract
Name
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Rizvi
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Ahmed
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1984-
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author
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Ahmed Rizvi
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Masayori
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chair
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Advisory Committee
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Masayori Inouye
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Ron
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Yacov
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internal member
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Advisory Committee
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Yacov Ron
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DiCicco-Bloom
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Emanuel
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internal member
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Advisory Committee
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Emanuel DiCicco-Bloom
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NamePart
Rutgers University
Role
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degree grantor
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Graduate School - New Brunswick
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school
OriginInfo
DateCreated
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2010
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2010
Place
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xx
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TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
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ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
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rucore19991600001
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NjNbRU
Identifier
(type = doi)
doi:10.7282/T3FT8M4P
Genre
(authority = ExL-Esploro)
ETD graduate
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Rights
RightsDeclaration
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The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
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(type = personal)
Name
FamilyName
Rizvi
GivenName
Ahmed
Role
Copyright Holder
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Type
Permission or license
DateTime
2010-03-12 04:43:54
AssociatedEntity
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Name
Ahmed Rizvi
Affiliation
Rutgers University. Graduate School - New Brunswick
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Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
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522240
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