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Regulation of yeast choline kinase by zinc depletion and the yeast phosphatidate phosphatase by zinc depletion and growth
Descriptive
TypeOfResource
Text
TitleInfo
(ID = T-1)
Title
Regulation of yeast choline kinase by zinc depletion and the yeast phosphatidate phosphatase by zinc depletion and growth
Identifier
ETD_2831
Identifier
(type = hdl)
http://hdl.rutgers.edu/1782.1/rucore10001600001.ETD.000056776
Language
LanguageTerm
(authority = ISO639-2);
(type = code)
eng
Genre
(authority = marcgt)
theses
Subject
(ID = SBJ-1);
(authority = RUETD)
Topic
Microbiology and Molecular Genetics
Subject
(ID = SBJ-2);
(authority = ETD-LCSH)
Topic
Saccharomyces cerevisiae
Subject
(ID = SBJ-3);
(authority = ETD-LCSH)
Topic
Phosphatidate phosphatase
Subject
(ID = SBJ-4);
(authority = ETD-LCSH)
Topic
Phospholipids
Subject
(ID = SBJ-5);
(authority = ETD-LCSH)
Topic
Zinc enzymes
Abstract
(type = abstract)
In the yeast Saccharomyces cerevisiae, the CKI1-encoded choline kinase catalyzes the committed step in the synthesis of phosphatidylcholine via the CDP-choline branch of the Kennedy pathway. Analysis of a PCKI1-lacZ reporter gene revealed that CKI1 expression was regulated by intracellular levels of the essential mineral zinc. Zinc depletion resulted in a concentration-dependent induction of CKI1 expression. This regulation was mediated by the zinc-sensing and zinc-inducible transcriptional activator Zap1p. A purified Zap1p probe interacted with two putative UASZRE sequences (ZRE1 and ZRE2) in the CKI1 promoter. Mutations of ZRE1 and ZRE2 to a nonconsensus UASZRE attenuated the induction of CKI1 expression in response to zinc depletion. A UASINO element in the CKI1 promoter was responsible for stimulating CKI1 expression, but this element was not involved in the regulation by zinc depletion. The induction of CKI1 expression in zinc-depleted cells translated into increased choline kinase activity in vitro and in vivo, and an increase in phosphatidylcholine synthesis via the Kennedy pathway. The yeast PAH1-encoded PA phosphatase (PAP) catalyzes the dephosphorylation of PA to yield DAG and Pi. The DAG produced by the PAP reaction is utilized for the synthesis of TAG and the synthesis of PE and PC via the Kennedy pathway. Analysis of the PPAH1-lacZ reporter gene demonstrated that PAH1 expression was regulated by intracellular levels of zinc. The induction of the PAH1 gene in response to zinc depletion was dependent on the concentration of zinc. PAP regulation by zinc depletion was mediated by the zinc responsive transcription factor Zap1p. Purified Zap1p interacted with three putative UASZRE sequences located in the PAH1 promoter. The Zap1p mediated induction in PAH1 expression in response to zinc depletion resulted in an increase in PAP activity in vitro. The induction in PAP activity in zinc-depleted cells translated into an increase in DAG and TAG levels. The pah1Δ mutation resulted in the decrease of PC synthesis via the Kennedy pathway in response to zinc depletion. The regulation of the yeast PAH1-encoded PAP by growth was examined. The DAG produced in the PAP reaction is converted into the storage lipid TAG throughout growth. As cells progress from the exponential to the stationary phase of growth the PAP activity is induced, resulting in an increased in DAG and TAG levels. The induction in PAP activity coincided with a decrease in PAP protein levels. PAP protein is degraded via the ubiquitin-proteasome pathway as cells progress throughout growth.
PhysicalDescription
Form
(authority = gmd)
electronic resource
Extent
xv, 160 p. : ill.
InternetMediaType
application/pdf
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text/xml
Note
(type = degree)
Ph.D.
Note
(type = bibliography)
Includes bibliographical references
Note
(type = vita)
Includes vita
Note
(type = statement of responsibility)
by Anibal Soto-Cardalda
Name
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(type = personal)
NamePart
(type = family)
Soto-Cardalda
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Anibal
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1980-
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author
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Anibal Soto-Cardalda
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NamePart
(type = family)
Carman
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(type = given)
George M
Role
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(authority = RULIB)
chair
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Advisory Committee
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George M Carman
Name
(ID = NAME-3);
(type = personal)
NamePart
(type = family)
Martin
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(type = given)
Charles E
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internal member
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Advisory Committee
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Charles E Martin
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NamePart
(type = family)
Kinzy
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(type = given)
Terri
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internal member
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Advisory Committee
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Terri Kinzy
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(ID = NAME-5);
(type = personal)
NamePart
(type = family)
Chikindas
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(type = given)
Mikhail
Role
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internal member
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Advisory Committee
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Mikhail Chikindas
Name
(ID = NAME-6);
(type = personal)
NamePart
(type = family)
Han
NamePart
(type = given)
Gil-Soo
Role
RoleTerm
(authority = RULIB)
outside member
Affiliation
Advisory Committee
DisplayForm
Gil-Soo Han
Name
(ID = NAME-1);
(type = corporate)
NamePart
Rutgers University
Role
RoleTerm
(authority = RULIB)
degree grantor
Name
(ID = NAME-2);
(type = corporate)
NamePart
Graduate School - New Brunswick
Role
RoleTerm
(authority = RULIB)
school
OriginInfo
DateCreated
(qualifier = exact)
2010
DateOther
(qualifier = exact);
(type = degree)
2010-10
Place
PlaceTerm
(type = code)
xx
RelatedItem
(type = host)
TitleInfo
Title
Rutgers University Electronic Theses and Dissertations
Identifier
(type = RULIB)
ETD
RelatedItem
(type = host)
TitleInfo
Title
Graduate School - New Brunswick Electronic Theses and Dissertations
Identifier
(type = local)
rucore19991600001
Location
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(displayLabel = Rutgers, The State University of New Jersey)
NjNbRU
Identifier
(type = doi)
doi:10.7282/T3XD11DS
Genre
(authority = ExL-Esploro)
ETD doctoral
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Rights
RightsDeclaration
(AUTHORITY = GS);
(ID = rulibRdec0006)
The author owns the copyright to this work.
Copyright
Status
Copyright protected
Availability
Status
Open
Reason
Permission or license
RightsHolder
(ID = PRH-1);
(type = personal)
Name
FamilyName
Soto-Cardalda
GivenName
Anibal
Role
Copyright Holder
RightsEvent
(ID = RE-1);
(AUTHORITY = rulib)
Type
Permission or license
DateTime
2010-08-30 16:57:43
AssociatedEntity
(ID = AE-1);
(AUTHORITY = rulib)
Role
Copyright holder
Name
Anibal Soto-Cardalda
Affiliation
Rutgers University. Graduate School - New Brunswick
AssociatedObject
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(AUTHORITY = rulib)
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License
Name
Author Agreement License
Detail
I hereby grant to the Rutgers University Libraries and to my school the non-exclusive right to archive, reproduce and distribute my thesis or dissertation, in whole or in part, and/or my abstract, in whole or in part, in and from an electronic format, subject to the release date subsequently stipulated in this submittal form and approved by my school. I represent and stipulate that the thesis or dissertation and its abstract are my original work, that they do not infringe or violate any rights of others, and that I make these grants as the sole owner of the rights to my thesis or dissertation and its abstract. I represent that I have obtained written permissions, when necessary, from the owner(s) of each third party copyrighted matter to be included in my thesis or dissertation and will supply copies of such upon request by my school. I acknowledge that RU ETD and my school will not distribute my thesis or dissertation or its abstract if, in their reasonable judgment, they believe all such rights have not been secured. I acknowledge that I retain ownership rights to the copyright of my work. I also retain the right to use all or part of this thesis or dissertation in future works, such as articles or books.
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Technical
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ETD
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application/pdf
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application/x-tar
FileSize
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1802240
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